AI Article Synopsis
- Chloroform shows covalent binding to DNA, RNA, and proteins in the organs of rats and mice after being injected, with RNA and proteins showing higher binding levels than DNA.
- In rat and mouse liver, chloroform is classified as a weak initiator based on its Covalent Binding Index values.
- The bioactivation of chloroform occurs via cytochrome P450 enzymes and GSH-transferases, particularly in the mouse lung, indicating that GSH is involved in how chloroform metabolites bind to DNA.
Article Abstract
Chloroform was bound covalently to DNA, RNA and proteins of rat and mouse organs in vivo after i.p. injection. Covalent Binding Index values of rat and mouse liver DNA classify chloroform as a weak initiator. Labelings of RNA and proteins from various organs of both species were higher than that of DNA. In an in vitro cell-free system, chloroform was bioactivated by cytochrome P450-dependent microsomal fractions, by cytosolic GSH-transferases from rat and mouse liver, and particularly by the latter enzymes from mouse lung. This observation suggests that GSH plays a role in the binding of chloroform metabolites to DNA. The presence of both microsomal and cytosolic enzymatic systems in the standard incubation mixture generally led to an additive or synergistic bioactivating effect for rat and mouse, respectively.
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| http://dx.doi.org/10.1177/030089169107700401 | DOI Listing |
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