AI Article Synopsis
- The study reveals that the ganglioside G(M1) leads to non-uniform distribution within lipid bilayers, indicating its tendency to self-aggregate as a natural property.
- Researchers used BODIPY-labelled gangliosides and conducted advanced fluorescence experiments to analyze these properties.
- They synthesized three types of fluorophore-labelled gangliosides to explore G(M1)'s behavior while ruling out any ganglioside-ganglioside interactions induced by the labels for future studies in more complex systems.
Article Abstract
We demonstrate that the ganglioside G(M1) in lipid bilayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) exhibits a non-uniform lateral distribution, i.e., enriched regions of GM(1) molecules are formed, which is an argument in favour of self-aggregation of G(M1) being an intrinsic property of G(M1) ganglioside. This was concluded from energy transfer/migration studies of BODIPY-labelled gangliosides by means of time-resolved fluorescence lifetime and depolarization experiments. Three fluorophore-labelled gangliosides were synthesized to include either of two spectroscopically different BODIPY groups. These were specifically localized either in the polar headgroup region or in the non-polar region of the lipid bilayer. An eventual ganglioside-ganglioside affinity/aggregation induced by the BODIPY groups was experimentally excluded, which suggests their use in examining the influence of G(M1) in more complex systems.
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| http://dx.doi.org/10.1080/09687860600995235 | DOI Listing |
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