We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2771206 | PMC |
| http://dx.doi.org/10.1038/nmeth1042 | DOI Listing |
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