Objective: To analyze the role of the local renin-angiotensin system (RAS) in the female reproductive system to modulate ovarian steroidogenesis and its relationship with alpha(1)adrenergic receptors.

Design: Observational study.

Setting: University laboratory.

Animal(s): Adult female Wistar rats treated with doxazosin (10 mg/kg) or vehicle for 15 days.

Intervention(s): Samples from the whole right ovary were dissected after perfusion with saline. The soluble and membrane-bound fractions were obtained from these samples. Also, blood samples were used to obtain the serum.

Main Outcome Measure(s): Fluorometric measurement of soluble and membrane-bound RAS-regulating proteolytic regulatory enzyme activities by using arylamide derivatives as substrates. Time-resolved fluoroimmunoassay of serum E(2) and P.

Result(s): alpha(1)Adrenergic receptor blockade increases ovarian soluble and membrane-bound aminopeptidase A and decreases membrane-bound aminopeptidase N and aminopeptidase B. Furthermore, serum P levels increased, whereas serum E(2) did not change.

Conclusion(s): Ovarian P production, at least in the rat, is regulated by noradrenaline through a mechanism of action in which the RAS is involved, with a main role for angiotensin III.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fertnstert.2006.12.021DOI Listing

Publication Analysis

Top Keywords

soluble membrane-bound
12
angiotensin iii
8
membrane-bound aminopeptidase
8
doxazosin blockade
4
blockade alpha
4
alpha 1-adrenergic
4
1-adrenergic receptors
4
receptors increases
4
increases rat
4
serum
4

Similar Publications

Vacuole fusion is driven by SNARE proteins that require activation-or priming-by the AAA+ protein Sec18 (NSF) before they can bring membranes together and trigger the merger of two bilayers into a continuous membrane. Sec18 resides on vacuoles prior to engaging inactive cis-SNARE complexes through its interaction with the regulatory lipid phosphatidic acid (PA). Binding PA causes Sec18 to undergo large conformational changes that keeps it bound to the membrane, thus precluding its interactions with SNAREs.

View Article and Find Full Text PDF

The Role of Fractalkine in Diabetic Retinopathy: Pathophysiology and Clinical Implications.

Int J Mol Sci

January 2025

Department of Ophthalmology, National Taiwan University Hospital, No. 7, Chung Shan S. Rd. (Zhongshan S. Rd.), Zhongzheng Dist., Taipei City 100225, Taiwan.

Diabetic retinopathy (DR) is a complication of diabetes, characterized by progressive microvascular dysfunction that can result in vision loss. Chronic hyperglycemia drives oxidative stress, endothelial dysfunction, and inflammation, leading to retinal damage and complications such as neovascularization. Current treatments, including anti-VEGF agents, have limitations, necessitating the exploration of alternative therapeutic strategies.

View Article and Find Full Text PDF

Soluble immune checkpoints (sIC) are crucial factors in the immune system. They regulate immune responses by transforming intercellular signals via binding to their membrane-bound receptor or ligand. Moreover, soluble ICs are vital in immune regulation, cancer development, and prognosis.

View Article and Find Full Text PDF

Soluble, circulating Klotho (sKlotho) is essential for normal health and renal function. sKlotho is shed from the renal distal convoluted tubule (DCT), its primary source, via enzymatic cleavage. However, the physiologic mechanisms that control sKlotho production, trafficking, and shedding are not fully defined.

View Article and Find Full Text PDF

Membrane-embedded CdaA is required for efficient synthesis of second messenger cyclic di-AMP.

Commun Biol

December 2024

Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Nijenborgh 3, Groningen, The Netherlands.

Cyclic di-adenylate monophosphate (cyclic di-AMP) is an important second messenger in microorganisms. Cyclic di-AMP regulates bacterial cell volume and turgor via control of potassium and compatible solute transport but is also involved in many other processes, including the activation of the metazoan innate immune response to bacterial infections. We compare the activity of full-length membrane-embedded CdaA, the enzyme that synthesizes cyclic di-AMP, with the water-soluble catalytic domain CdaA-DAC.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!