A novel multi-array immunoassay device based on the insert-plug model of piezoelectric (Pz) immunosensor fabricated with the screw clamp apparatus has been developed for quantitative detection of tumor markers such as alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), prostate specific antigen (PSA), and carcinoma antigen 125 (CA125) in serum, in which single immunosensor can oscillate independently with the frequency stability of +/-1 Hz (hertz) in air phase and +/-2 Hz in liquid phase. These response characteristics of Pz tumor marker multi-array immunoassay device such as time-cost, reproducibility and specificity, etc. were also investigated, respectively. The detection range for AFP, CEA, PSA and CA125 obtained by multi-array Pz immunosensor were 20-640 ng/ml, 1.5-30 microg/ml, 1.5-40 ng/ml and 5-150 IU/ml, respectively, with the coefficient of variance (CV) less than 5% and no cross-reactivates with other tumor markers in serum were observed. Application of the multi-array immunosensor to clinical samples demonstrated that results were in good agreement with chemiluminescence immunoassay (CLIA). Moreover, the multi-array Pz immunosensor could be regenerated to be reused for three cycles without appreciable loss of response activity. Therefore, the proposed multi-array immunoassay device based on Pz immunosensor provides a rapid, sensitive, specific, reusable, convenient and reliable alternative for the detection of tumor markers in clinical laboratory.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bios.2007.03.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nanozyme linked multi-array gas driven sensor for real-time quantitative detection of .
Analyst
November 2024
Department of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
() is a pathogen typically transmitted through respiratory droplets and skin contact, causing an estimated 700 million mild non-invasive infections worldwide each year. There are approximately 650 000 infections that progress to severe invasive infections, even resulting in death. Therefore, the ability to detect rapidly, accurately and in real time is important.
View Article and Find Full Text PDFFast and Efficient Measurement of Clinical and Biological Samples Using Immunoassay-Based Multiplexing Systems.
Methods Mol Biol
October 2020
Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA, USA.
Immunoassay is one of the most commonly used biomedical techniques to detect the expression of an antibody or an antigen in a test sample. Enzyme-linked immunosorbent assay (ELISA) has been used for a variety of applications including diagnostic tools and quality controls. However, one of the main limitations of ELISA is its lack of multiplexing ability, so ELISA may not be an efficient diagnostic tool when a measurement of multiple determinants is needed for samples with limited quantity such as blood or biological samples from newborns or babies.
View Article and Find Full Text PDFGalectin-3 and aldosterone as potential tandem biomarkers in pulmonary arterial hypertension.
Heart
March 2016
Department of Pediatric Cardiology and Critical Care, Hannover Medical School, Hannover, Germany.
Background: Several studies have identified circulating biomarkers to be associated with the presence and severity of pulmonary arterial hypertension (PAH). Recent evidence supports a role for galectin-3 (Gal-3) and the mineralcorticoid aldosterone in left ventricular failure. However, studies on aldosterone together with Gal-3 in PAH are lacking.
View Article and Find Full Text PDFA microfluidic multiplex proteomic immunoassay device for translational research.
Clin Proteomics
December 2015
Department of Laboratory Medicine and Pathology, University of Minnesota, Twin Cities, 420 Delaware Street SE, MMC 609, Minneapolis, MN 55455 USA.
Objective: Microfluidic technology has the potential to miniaturize and automate complex laboratory procedures. The objective of this study was to assess a microfluidic immunoassay device, Simple Plex, which simultaneously measured IL-1β, TNF-α, IL-6, and IL-10 in serum samples. This assessment is important to understanding the potentials of this microfluidic device as a valuable tool in translational research efforts.
View Article and Find Full Text PDFEvaluation and validation of biochip multi-array technology for the screening of six families of antibiotics in honey according to the European guideline for the validation of screening methods for residues of veterinary medicines.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
June 2015
a ANSES, Laboratoire de Fougères , Fougères Cedex , France.
The main chemicals used against varoa are acaricides, and the antibiotics used for the control of bee bacterial diseases are mainly tetracyclines, streptomycins, sulfonamides and chloramphenicol. No maximum residue limits (MRLs) have been set for any antibiotics in honey. Therefore, in the European Union, minimum recommended concentrations (RC) for the analytical performance of methods to control a certain set of these non-authorised chemicals in honey were published by the European Union Reference Laboratory (EU-RL) in 2007.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!