AI Article Synopsis
- The study aimed to analyze the genetic variations at five Short Tandem Repeat (STR) loci in the Chengdu Han population to create a forensic database.
- Blood samples from unrelated individuals were collected and analyzed using techniques like PCR amplification and gel electrophoresis to identify alleles and genetic polymorphisms.
- Results showed high heterozygosity and discrimination power at all five loci, indicating their usefulness in forensic applications and contributing to population genetics research.
Article Abstract
Objective: To understand the allele structure and genetic polymorphisms at five STR loci in Chengdu Han population, to obtain a preliminary database and to analyze the value in forensic medicine.
Methods: EDTA-blood specimens were collected from unrelated individuals. The DNA of samples was extracted with Chelex-100 method. And then the PCR amplification, PAGE horizontal electrophoresis and gel silver staining were performed for analyzing the polymorphisms of five STR loci in Chengdu Han population.
Results: In the five STR loci,10, 7, 5, 7 and 7 alleles were found respectively. The observed heterozygosity of 83%, 80%, 64%, 66% and 77% and discrimination power of 95.44%, 91.0%, 84.3%, 86.1% and 86.8% were identified for the five STR loci respectively. All loci obeyed Hardy-Weinberg equilibrium.
Conclusion: The five STR loci display the high discrimination power. The obtained data are desirable for forensic analysis and benefiting to the research of the population genetics.
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