Sensitive, specific, and accurate methods to assay chemosensitivity are needed to (1) screen new therapeutic agents, (2) identify patterns of chemosensitivity for different tumor types, (3) establish patterns of cross-resistance and sensitivity in treatment of naïve and relapsing tumors, (4) identify genomic and proteomic profiles associated with sensitivity, (5) correlate in vitro response with preclinical in vivo effects and clinical outcomes for a particular therapeutic agent, and (6) tailor chemotherapy regimens to individual patients. Various methods are available to achieve these end points, including several in vitro clonogenic and proliferation assays, cell metabolic activity assays, molecular assays to monitor expression of markers for responsiveness, drug resistance, and for induction of apoptosis, in vivo tumor growth and survival assays in metastatic and orthotopic models, and in vivo imaging assays. The advantages and disadvantages of the specific assays are discussed. A summary of research questions related to chemosensitivity testing is also included.
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http://dx.doi.org/10.1007/BF02686104 | DOI Listing |
Pathol Int
December 2024
Department of Pathology, Kitasato University School of Medicine, Sagamihara, Japan.
REV7 is a multifunctional protein essential for promoting cellular tolerance to DNA damage. REV7 expression is associated with disease progression and prognosis in several human malignant tumors. This study aimed to evaluate the clinical and biological significance of REV7 in gastric adenocarcinoma (GAD).
View Article and Find Full Text PDFPhysiol Rep
December 2024
Department of Kinesiology and Health Sciences, University of Waterloo, Waterloo, Ontario, Canada.
At rest, the menstrual cycle phase impacts ventilation and chemosensitivity. However, during exercise there is inconclusive evidence that the menstrual cycle phase affects ventilation or chemosensitivity. We sought to examine the influence of menstrual phase and hormonal birth control (BC) on chemosensitivity.
View Article and Find Full Text PDFMol Metab
December 2024
Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy. Electronic address:
J Cell Mol Med
December 2024
Graduate Institute of Biomedical Sciences, Program for MD/PhD, Research Center for Cancer Biology, School of Medicine, China Medical University, Taichung, Taiwan.
Discov Nano
December 2024
Department of Oncology, The First People's Hospital of Yancheng City, The Yancheng Clinical College of Xuzhou Medical University, Yancheng, 224005, Jiangsu, People's Republic of China.
Cisplatin (CDDP) is the primary drug used in the initial treatment of esophageal cancer (EC). However, its side effects and resistance can limit its effectiveness in clinical therapy. Curcumin (Cur)-mediated glutathione (GSH) depletion can reverse resistance, enhance the chemosensitivity of CDDP, and further improve the efficacy of platinum-containing chemotherapy in the treatment of esophageal cancer.
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