Background & Objective: Melanoma differentiation associated gene-7 (mda-7/IL-24) has double functions: specifically induces tumor cell apoptosis and modulates immune responses. Therefore, it is a strong candidate for human cancer gene therapy. This study was to evaluate the effect of adenovirus-mediated mda-7/IL-24 infection on the apoptosis of drug-resistant ovarian cancer cell lines OVCAR-3 and OVCAR-8/TR.
Methods: Adenovirus-mediated mda-7/IL-24 (Ad.mda-7/IL-24) was constructed using AdEasy 1 system. OVCAR-3 and OVCAR-8/TR cells were infected by Ad.mda-7/IL-24. The expression of MDA-7/IL-24 protein was detected by Western blot. Cell apoptosis was detected by flow cytometry with Hoechst33258 staining. Cell cycle distribution was detected by flow cytometry.
Results: The recombinant Ad.mda-7/IL-24 was confirmed by DNA sequencing and electrophoresis. The expression of MDA-7/IL-24 protein was detected in the cells after infection. Within 72 h after Ad.mda-7/IL-24 infection, the maximal apoptosis rates of OVCAR-3 and OVCAR-8/TR cells were 14.1% and 32.4%, respectively, significantly higher than empty vector group and uninfected group.
Conclusions: The recombinant Ad.mda-7/IL-24 was successfully constructed. It can induce apoptosis in drug-resistant ovarian cancer OVCAR-3 and OVCAR-8/TR cells.
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