Objective: To construct and express anti-human RBC and HIVgp160 fusion protein for rapid detection of antibody to HIV.
Methods: The gene of the anti human RBC ScFv and HIV antigen were constructed together into expression vector. The fusion protein was expressed in E. coli.
Results: The fusion protein was proved to be able to bind both anti-RBC and HIVgp160. It could cause agglutination of human RBC when HIVgp160 was present.
Conclusion: The fusion protein has the potential in rapid detection of HIV.
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