Objective: To probe into the feasibility of screening anti-HIV compounds by using HIV-1 p24 detection kit made by Hebei Medical University.
Methods: The sensitivity, reproducibility and efficacy of the Hebei p24 kit were evaluated compared with the commercially available Vironostika HIV-1 Antigen Microelisa System (Biomerieux).
Results: Hebei p24 kit had high sensitivity and good reproducibility. In vitro screening demonstrated that there was no statistically significant difference (P greater than 0.05) between these two kits in assessing anti-HIV compounds.
Conclusion: Hebei p24 kit could be used as an easily affordable alternative method for detection of HIV-1 in screening anti-HIV compounds.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Comparison: Imported and Indigenous HIV Fourth-generation immunodiagnostic kits.
Asian J Transfus Sci
December 2024
Immunodiagnostic Kit Laboratory, National Institute of Biologicals, Noida, Uttar Pradesh, India.
Early detection of any disease is always a life-saving methodology for human beings likewise the detection of p24 antigen is always better than the detection of HIV antibodies. In the current era, p24 antigen is added additionally in HIV antibodies detection assays and called HIV fourth-generation immunodiagnostic kit. The different HIV fourth-generation kit having different capacities to pick up the low concentration of analyte as well as represent the detection values in different units.
View Article and Find Full Text PDFInhibition of HIV-1 infection with curcumin conjugated PEG-citrate dendrimer; a new nano formulation.
BMC Complement Med Ther
October 2024
Department of Medical Lab Technology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Article Synopsis
- Scientists are studying a special tiny delivery system called dendrimers to help get more effective medicine into cells, like curcumin, which can help fight viruses like HIV-1.
- They created a new type of dendrimer that can make curcumin more dissolvable in water and easier for cells to absorb.
- The tests showed that this curcumin-dendrimer combination can significantly stop HIV from growing, making it a strong candidate for future treatments.
Development of a novel monoclonal antibody-based competitive ELISA for antibody detection against bovine leukemia virus.
Int J Biol Macromol
May 2024
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China. Electronic address:
Infection with bovine leukemia virus (BLV) leads to enzootic bovine leukosis, the most prevalent neoplastic disease in cattle. Due to the lack of commercially available vaccines, reliable eradication of the disease can be achieved through the testing and elimination of BLV antibody-positive animals. In this study, we developed a novel competitive ELISA (cELISA) to detect antibodies against BLV capsid protein p24.
View Article and Find Full Text PDFAnalytical and clinical performance evaluation of enhanced chemiluminescence-based fourth-generation HIV combo assay: Report from tertiary health-care setup in North India.
Asian J Transfus Sci
May 2023
Indepedent Laboratory Consultant, Chennai, Tamil Nadu, India.
Introduction: HIV fourth-generation assay, designed for the detection of HIV p24 antigen along with anti-HIV antibodies of both immunoglobulin M and immunoglobulin G type against HIV 1 and HIV 2 viral antigens, have helped in the early detection of HIV infection and supports in minimizing the transmission risk in the acute phase of infection. The objective of this study was to evaluate the analytical and clinical performance of HIV fourth-generation assay based on enhanced chemiluminescence technology.
Materials And Methods: The analytical performance of the assay was evaluated in terms of accuracy, precision, limit of detection, type of sample (serum vs.
Field Performance of a Rapid Test to Detect Progressive, Regressive, and Abortive Feline Leukemia Virus Infections in Domestic Cats in Australia and Germany.
Viruses
February 2023
Clinical Laboratory, Department of Clinical Diagnostics and Services, and Center for Clinical Studies, Vetsuisse Faculty, The University of Zurich, CH-8057 Zurich, Switzerland.
Different feline leukemia virus (FeLV) infection outcomes are possible in cats following natural exposure, such as progressive infections (persistent viremia), regressive infections (transient or no viremia followed by proviral persistence) and abortive infections (presence of only antibodies). Laboratory-based testing is currently required for categorization of infection outcomes in cats. The aim of this study was to evaluate the field performance of a novel, rapid, combination point-of-care (PoC) test kit commercially available in Europe (v-RetroFelAg/Ab; 2020-2021 version) to determine different FeLV infection outcomes by concurrent detection of FeLV antigen (p27) and antibodies against FeLV transmembrane envelope protein (p15E).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!