AI Article Synopsis
- Bone marrow-derived stem cells are considered promising for liver gene therapy and regenerative medicine, but their behavior in liver transplants is not fully understood.
- A study analyzed liver biopsies from nine sex-mismatched transplant recipients to track the presence of recipient-derived hepatocytes over time, using advanced imaging techniques.
- Results showed a significant decrease in recipient-derived hepatocyte repopulation from the first biopsy (2.0%) to the second biopsy (0.5%), indicating that this repopulation occurs mainly early after transplantation.
Article Abstract
Background: ''Bone marrow-derived stem cells'' have attracted great attention as potential candidates for liver-directed gene therapy and as a tool for regenerative medicine. However, the fate of these cells is not well-known. The aim of this present study was to investigate the fate of ''recipient-derived repopulated hepatocytes'' in sex-mismatched liver allografts in individuals following liver transplantation during systematic longitudinally performed liver biopsies.
Methods: Paraffin-embedded sex-mismatched liver biopsy samples of nine recipients (male/female ratio 5/4; mean age: 39.7 yr) were reviewed. Double labeling with immunohistochemistry for hepatocytes and recipient-specific bone marrow-derived cells and fluorescence in-situ hybridization for visualizing X and Y chromosomes were performed. These slides were examined systematically using an image analyzer system (Olympus microscope; Cyto-Vision, Applied Imaging, Biosciences Centre, Newcastle, UK). Only cells with two nuclear spots were considered for interpretation.
Results: The mean times from transplantation to first biopsy and between the first and the second biopsies were 5.9 and 20.9 months respectively. The proportion of recipient-derived repopulated hepatocytes was significantly decreased in the late biopsies when compared with the early biopsies (p = 0.001). All nine samples of the first biopsies had demonstrated recipient-derived hepatocyte repopulation, with a mean of 2.0%, whereas only seven of nine samples of the second biopsies had demonstrated recipient-derived hepatocyte repopulation with a low mean of 0.5% (p = 0.001).
Conclusion: Based on these results, we suggest that ''recipient-specific bone marrow-derived hepatocyte repopulation'' in liver allograft during tissue injury is a relatively early event.
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| http://dx.doi.org/10.1111/j.1399-0012.2006.00623.x | DOI Listing |
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