[Study on inducing differentiation of mouse embryonic stem cells into cardiomyocyte in vitro].

Objective: To study the differentiation from embeyonic stem cells into cardiomyocyte.

Methods: Gene alpha/ beta-MHC, a specific gene of cardiomyocyte and another three house-keeping genes were detected by RT-PCR from the ESCs of 0-,2-,4-,6-,8-, 10-,12-,14-,16-,18- and 20 day and ESCs of 0-,3-, 5- and 10 day with specific culture condition.

Results: ESCs started to express alpha/betaMHC gene from 6 day and the levels of alpha/betaMHC increased with culture time. The levels of 14 day's alpha/betaMHC were the highest. After 14 day, alpha/betaMHC gene kept the stable levels. The levels of three house-keeping genes changed significantly with culture time, and GAPDH kept the best stability of three genes.

Conclusion: The cycle of the differentiation from ESCs into cardiomyocyte in vitro could be designed as 10 - 12 days and GAPDH could be selected as the fittest inner comparison.