This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.
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Development and validation of a UV-Vis spectrophotometric method for estimation of total content of chalcone.
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Observatory of Drug-Herb Interactions, Faculty of Pharmacy, University of Coimbra, Health Science Campus, Azinhaga Santa Comba, Coimbra, Portugal.
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Lab of Hepatopharmacology and Ethnopharmacology, School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, China.
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Department of Biology, College of Science, Bahir Dar University, P. O. Box 79, Bahir Dar, Ethiopia.
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