Molecular analysis of genes in Nostoc punctiforme involved in pilus biogenesis and plant infection.

J Bacteriol

Institute of Integrative and Comparative Biology, Garstang Building, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, United Kingdom.

Published: June 2007

Hormogonia are the infective agents in many cyanobacterium-plant symbioses. Pilus-like appendages are expressed on the hormogonium surface, and mutations in pil-like genes altered surface piliation and reduced symbiotic competency. This is the first molecular evidence that pilus biogenesis in a filamentous cyanobacterium requires a type IV pilus system.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1913353PMC
http://dx.doi.org/10.1128/JB.01927-06DOI Listing

Publication Analysis

Top Keywords

pilus biogenesis
8
molecular analysis
4
analysis genes
4
genes nostoc
4
nostoc punctiforme
4
punctiforme involved
4
involved pilus
4
biogenesis plant
4
plant infection
4
infection hormogonia
4

Similar Publications

c-di-GMP is an important second messenger in bacteria regulating, for example motility, biofilm formation, cell wall biosynthesis, infectivity, and natural transformability. It binds to a multitude of intracellular receptors. This includes proteins containing general secretory pathway II (GSPII) domains such as the N-terminal domain of the Vibrio cholerae ATPase MshE (MshEN) which binds c-di-GMP with two copies of a 24-amino acids sequence motif.

View Article and Find Full Text PDF

is an emerging foodborne pathogen. We previously reported that some avian Shiga toxin-producing strains exhibited higher or comparable cytotoxicity in Vero-d2EGFP cells with several enterohemorrhagic (EHEC) outbreak strains. To better understand the environmental persistence of this pathogen, comparative genomics and phenotypic assays were applied to assess adhesion capability, motility, and biofilm formation in .

View Article and Find Full Text PDF

Gram-negative bacteria produce chaperone-usher pathway pili, which are extracellular protein fibers tipped with an adhesive protein that binds to a receptor with stereochemical specificity to determine host and tissue tropism. The outer-membrane usher protein, together with a periplasmic chaperone, assembles thousands of pilin subunits into a highly ordered pilus fiber. The tip adhesin in complex with its cognate chaperone activates the usher to allow extrusion across the outer membrane.

View Article and Find Full Text PDF

Biogenesis and Functionality of Sortase-Assembled Pili in Gram-Positive Bacteria.

Annu Rev Microbiol

November 2024

Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California, USA.

Article Synopsis
  • Pili, or fimbriae, are unique proteins made of linked subunits that are found on the surface of gram-positive bacteria, assembled by an enzyme called sortase.
  • These proteins are important for a variety of functions, including adhesion to surfaces, immune system interaction, and roles in disease-causing abilities of bacteria.
  • The review discusses ongoing research into how pili are formed, their functions, potential applications in vaccines, and the future of this area of study.
View Article and Find Full Text PDF

Type 1 fimbria and P pili: regulatory mechanisms of the prototypical members of the chaperone-usher fimbrial family.

Arch Microbiol

August 2024

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Mor, 62210, Mexico.

Adherence to both cellular and abiotic surfaces is a crucial step in the interaction of bacterial pathogens and commensals with their hosts. Bacterial surface structures known as fimbriae or pili play a fundamental role in the early colonization stages by providing specificity or tropism. Among the various fimbrial families, the chaperone-usher family has been extensively studied due to its ubiquity, diversity, and abundance.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!