AI Article Synopsis
- Microwave irradiation speeds up solid-phase peptide synthesis, making the process faster and yielding purer products.
- Using 96-well polypropylene filter plates allows for efficient parallel synthesis of peptide libraries without the need for extensive purification methods like HPLC.
- The protocol enables the synthesis of various peptides in a short time, producing a library of 96 different hexapeptides in just 24 hours with decent initial purity and yield.
Article Abstract
The application of microwave irradiation to solid-phase peptide synthesis increases product purity and reduces reaction time. Parallel synthesis in 96-well polypropylene filter plates with microwave irradiation is an efficient method for the rapid generation of combinatorial peptide libraries in sufficient purity to assay the products directly for biological activity without HPLC purification. In this protocol, the solid-phase support is arrayed into each well of a 96-well plate, reagents are delivered using a multichannel pipette and a microwave reactor is used to complete peptide coupling reactions in 6 min and Fmoc-removal reactions in 4 min under temperature-controlled conditions. The microwave-assisted parallel peptide synthesis protocol has been used to generate a library of difficult hexa-beta-peptides in 61% average initial purity (50% yield) and has been applied to the preparation of longer alpha- and beta-peptides. Using this protocol, a library of 96 different hexapeptides can be synthesized in 24 h (excluding characterization).
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| http://dx.doi.org/10.1038/nprot.2007.23 | DOI Listing |
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