Although the fractured aquifer of the Salento supplies over 80% of the drinking water requirements of the local population, its exposure to pollution has recently increased. In recent years, owing to the arid climate and droughts, the spreading of wastewater on soil for irrigation has become much more frequent. Consequently, hazardous and pathogenic microorganisms released with wastewater have been transported into the subsoil and have contaminated groundwater. An elaboration of epidemiological data has shown that the local population has the highest exposure to endemic gastroenteritis in Italy. In order to reduce human exposure to unsafe groundwater, the setback distance for drinking wells necessary to achieve the 'natural disinfection' criteria, has been determined experimentally at the Nardò aquifer (Salento region), supported by groundwater monitoring results and a mathematical transport model able to determine the apparent pathogenic microorganism pathways in fractures. The results also provided valuable inactivation constants of cultural indicators (coliforms, enterococci, Clostridium spores and somatic coliphages) and viruses in the wastewater that have been injected into the fractured aquifer since 1991. Furthermore, the efficacy of chlorine to remove viral indicators from water in a well 500 m from wastewater injection was tested. Hypochlorination reduces somatic coliphages and Clostridium spores in groundwaters but did not achieve complete inactivation in all tests. Complete disinfection of groundwater samples was possible only when there was an initial Clostridium spores count of < or = 10 CFU 100 ml (-1).
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http://dx.doi.org/10.2166/wh.2006.054 | DOI Listing |
Front Microbiol
December 2024
Oniris VetAgroBio, INRAE, SECALIM, Nantes, France.
Our study aims to assess the thermal inactivation of non-proteolytic type B spores in a plant-based fish and to evaluate the potential of alternative heat treatments at temperatures below the safe harbor guidelines established for vacuum-packed chilled products of extended durability. First, the heat resistance of the spore suspension was determined using capillary tubes in potassium phosphate buffer at 80°C. The D value was estimated to be 0.
View Article and Find Full Text PDFAust Vet J
December 2024
Centre for Veterinary Education, Sydney School of Veterinary Science, The University of Sydney, Sydney, Australia.
A 12-year-old Bengal cat with unclassified cardiomyopathy presented for signs consistent with aortic thromboembolism (ATE). It was treated with clopidogrel and rivaroxaban as antithrombotic therapy, combined with narcotic analgesia and frusemide. After initially making a clinical improvement, the cat's condition suddenly deteriorated, with a presumptive diagnosis of clostridial myonecrosis.
View Article and Find Full Text PDFBMC Complement Med Ther
December 2024
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Background: A precise observation is that the cervix's solid tumors possess hypoxic regions where the oxygen concentration drops below 1.5%. Hypoxia negatively impacts the host's immune system and significantly diminishes the effectiveness of several treatments, including radiotherapy and chemotherapy.
View Article and Find Full Text PDFTer Arkh
December 2024
Izhevsk State Medical Academy.
The article is devoted to a form of botulism that has been little studied in our country and is registered exclusively in infants. The fundamental difference between this form and the most common foodborne botulism is that infants become infected by ingestion of spores, followed by their germination, colonization of the intestines and production of botulinum toxin , which leads to the development of life-threatening flaccid paralysis. Taking into account the peculiarities of pathogenesis, the clinical manifestations of infant botulism have some features, which are discussed by the authors.
View Article and Find Full Text PDFPLoS Biol
December 2024
University of Würzburg, Faculty of Medicine, Institute of Molecular Infection Biology, Würzburg, Germany.
Bacterial noncoding RNAs fulfill a variety of cellular functions as catalysts, as scaffolds in protein complexes or as regulators of gene expression. They often exhibit complex tertiary structures that are a key determinant of their biochemical function. Here, we characterize the structured "raiA motif" RNA from Clostridioides difficile, which is conserved in more than 2,500 bacterial species from the phyla Bacillota and Actinomycetota.
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