The state of adsorbed protein molecules can be examined by comparing the shifts in a narrow line resonance wavelength of transverse electric (TE) and transverse magnetic (TM) whispering gallery modes (WGM) when the molecules adsorb onto a transparent microsphere that houses WGM. In adsorption of bovine serum albumin (BSA) onto an aminopropyl-modified silica microsphere, the TM/TE shift ratio indicated highly anisotropic polarizability of BSA in the direction normal to the surface, most likely ascribed to anchoring the heart-shaped protein molecule by one of its tips. The polarization-dependent resonance shift was confirmed when the surrounding refractive index was uniformly changed by adding salt, which would simulate adsorption of large objects.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1877779 | PMC |
| http://dx.doi.org/10.1529/biophysj.106.103200 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microfluidic-assisted sol-gel preparation of monodisperse mesoporous silica microspheres with controlled size, surface morphology, porosity and stiffness.
Nanoscale
January 2025
National Engineering Research Center for Colloidal Materials, School of Chemistry & Chemical Engineering, Shandong University, Jinan 250100, P. R. China.
The controllable synthesis of monodisperse mesoporous silica microspheres with unique physicochemical properties is becoming increasingly important for a variety of applications such as catalysts, chromatography, drug delivery and sensors. Here, we report a facile microfluidic-assisted sol-gel method for the preparation of silica microspheres with precisely controlled properties such as the size of the microspheres, the surface morphology, porosity and stiffness. All these properties can be manipulated by changing specific synthesis parameters, such as changing the microfluidic channels to tune the size of the microdroplets (tens to hundreds of microns), changing the contents of the precursor solution to manipulate the surface morphology (wrinkled to smooth surface) and changing the gelation/annealing conditions to tune the porosity (surface area up to 1021 m g) and stiffness of the microspheres (elastic modulus tunable from 0.
View Article and Find Full Text PDFUltrasensitive point-of-care multiplex diagnosis for influenza virus based robust quantum dot microsphere-lateral flow immunoassay.
Biosens Bioelectron
January 2025
Key Lab for Special Functional Materials of Ministry of Education, and School of Nanoscience and Materials Engineering, Henan University, 475004, Kaifeng, China. Electronic address:
Influenza A virus (IAV) and influenza B virus (IBV) with similar symptoms of infection caused a serious disease burden and economic losses in annual epidemic season, so it is important to quickly and accurately detect and distinguish between IAV and IBV during influenza season. Herein, the quantum dot microspheres (QDMS) were synthesized and applied to lateral flow immunoassays (LFIA), and a point-of-care (POC) biosensor that can discriminately and simultaneously diagnose IAV and IBV within 10 min was established. A double-sandwich QDMS nanotags was synthesized by immobilizing hydrophobic quantum dots (QDs) with chemical bonding method on a silica sphere template with an outer silica shell protection showed excellent stability and high fluorescence.
View Article and Find Full Text PDFDual surrogate imprinting: an innovative strategy for the preparation of virus-selective particles.
Mater Horiz
January 2025
Institute of Analytical and Bioanalytical Chemistry, Ulm University, 89081, Ulm, Germany.
This work involves the preparation of dual surrogate-imprinted polymers (D-MIPs) for the capture of SARS-CoV-2. To achieve this goal, an innovative and novel dual imprinting approach using carboxylated-polystyrene (PS-COOH) nanoparticles with a diameter of 100 nm and a SARS-CoV-2 Spike-derived peptide was carried out at the surface of amine-functionalized silica (PS-NH) microspheres with a diameter of 500 nm. Firstly, PS-COOH nanoparticles with the same size and spherical shape as the SARS-CoV-2 virus were employed to form hemispherical indentations (HI) at the surface of the PS-NH microspheres (obtaining dummy particle-imprinted polymers, HI-MIPs).
View Article and Find Full Text PDFUltrafast humidity sensor and transient humidity detections in high dynamic environments.
Commun Eng
January 2025
The School of Information Science and Technology, Southwest Jiaotong University, Chengdu, China.
Limited by the adsorption and diffusion rate of water molecules, traditional humidity sensors, such as those based on polymer electrolytes, porous ceramics, and metal oxides, typically have long response times, which hinder their application in monitoring transient humidity changes. Here we present an ultrafast humidity sensor with a millisecond-level response. The sensor is prepared by assembling monolayer graphene oxide quantum dots on silica microspheres using a simple electrostatic self-assembly technique.
View Article and Find Full Text PDFSelf-Healing Superhydrophobic Coatings with Multiphase Repellence Property.
ACS Appl Mater Interfaces
January 2025
Centre for Advanced Laser Manufacturing (CALM), School of Mechanical Engineering, Shandong University of Technology, Zibo 255000, P. R. China.
Developing versatile, scalable, and durable coatings that repel various matters in different service environments is of great importance for engineered materials applications but remains highly challenging. Here, the mesoporous silica microspheres (HMS) fabricated by the hard template method were utilized as micro-nanocontainers to encapsulate the hydrophobic agent of perfluorooctyltriethoxysilane (F13) and the corrosion inhibitor of benzotriazole (BTA), forming the functional microsphere of F-HMS(BTA). Moreover, the synthesized organosilane-modified silica sol adhesive (SMP) and F-HMS(BTA) were further employed as the binder and functional filler to construct a superhydrophobic self-healing coating of SMP@F-HMS(BTA) on various engineering metals through scalable spraying.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!