The checkpoint protein Rad9/Rad1/Hus1 heterotrimer (the 9-1-1 complex) is structurally similar to the proliferating cell nuclear antigen sliding clamp and has been proposed to sense DNA damage that leads to cell cycle arrest or apoptosis. Human (h) NEIL1 DNA glycosylase, an ortholog of bacterial Nei/Fpg, is involved in repairing oxidatively damaged DNA bases. In this study, we show that hNEIL1 interacts with hRad9, hRad1 and hHus1 as individual proteins and as a complex. Residues 290-350 of hNEIL1 are important for the 9-1-1 association. A significant fraction of the hNEIL1 nuclear foci co-localize with hRad9 foci in hydrogen peroxide treated cells. Human NEIL1 DNA glycosylase activity is significantly stimulated by hHus1, hRad1, hRad9 separately and the 9-1-1 complex. Thus, the 9-1-1 complex at the lesion sites serves as both a damage sensor to activate checkpoint control and a component of base excision repair.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885643 | PMC |
| http://dx.doi.org/10.1093/nar/gkm075 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Intraoperative HIFU ablation of the liver at the hepatocaval confluence as adjunct to surgery: Preliminary animal experiments.
Ultrasonics
December 2024
LabTAU, INSERM, Centre Leon Berard, Universite Lyon 1, F-69003 LYON, France. Electronic address:
Treating colorectal liver metastases (CLMs) located at the hepatocaval confluence with surgery is challenging due to its complexity and associated high risks of perioperative mortality and morbidity. Moreover, thermal ablation techniques are sensitive to the "heat-sink" effect, which reduces their efficacy when tumors are in contact with major blood vessels. In this study we evaluated the feasibility and safety of an intraoperative high-intensity focused ultrasound (HIFU) device for destroying liver tissue volumes sufficiently large to consider treating CLMs at the hepatocaval confluence.
View Article and Find Full Text PDF[Achondroplasia : New era of orthopedic treatment?].
Orthopadie (Heidelb)
December 2024
Abteilung für Kinderorthopädie und Fußchirurgie, Orthopädisches Spital Speising, Speisinger Str. 109, 1130, Wien, Österreich.
Achondroplasia is the most common skeletal dysplasia. With the development of new growth-promoting drug treatment for children and adolescents with achondroplasia, multidisciplinary care has become increasingly more important. In addition to orthopedic care, a specialized team comprised of pediatrics/endocrinology, radiology, neurosurgery, otorhinolaryngology, anesthesiology, physiotherapy, psychology and other disciplines is necessary to develop and implement a holistic concept to improve the quality of life for individuals affected by achondroplasia.
View Article and Find Full Text PDFNew Minimally Invasive Method for Treating Posterior Pelvic Ring Fractures: Biomechanical Validation and Clinical Application of Sacroiliac Joint Locking Plate.
Orthop Surg
November 2024
Department of Orthopedic Surgery, Nanping First Hospital Affiliated to Fujian Medical University, Nanping, China.
Objective: Considering the high incidence and complexity of unstable posterior pelvic ring fractures, and the need for less invasive and more effective treatment options, this study aims to introduce a novel minimally invasive, safe, and simple internal fixation method for the treatment of unstable posterior pelvic ring fractures using the sacroiliac joint locking plate (SJP) system, and to provide biomechanical validation and clinical evaluation of this method.
Methods: Biomechanical research was conducted using standard pelvic bone models from Synbone, Switzerland, to create Denis II zone unstable posterior pelvic ring fracture models, and to assess the stability of the SJP under different loads compared with traditional fixation methods. A retrospective clinical study analyzed the clinical efficacy of SJP treatment in 62 patients (mean age of 51.
Differences in primary total shoulder arthroplasty volume, reimbursement, practice styles, and patient populations based on surgeon gender: a temporal analysis.
J Shoulder Elbow Surg
October 2024
Department of Orthopedic Surgery, Mayo Clinic, Phoenix, AZ, USA.
Background: Orthopedic surgery has previously been shown to have a shortage of female physicians and a gender pay gap. However, this has not been thoroughly evaluated in the setting of shoulder surgery. The primary purpose of this study was to evaluate differences in total shoulder arthroplasty (TSA) volume, reimbursement, surgeon billing practices, and patient populations between male and female surgeons from 2013 to 2021.
View Article and Find Full Text PDFApplication of the "Klotski Technique" in Cervical Ossification of the Posterior Longitudinal Ligament With En Bloc Type Dura Ossification.
Neurospine
September 2024
Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.
Article Synopsis
- The anterior controllable antedisplacement and fusion (ACAF) technique, particularly the "Klotski technique," presents a new approach for treating cervical ossification of the posterior longitudinal ligament (OPLL) while minimizing fused segments.
- A study of 25 patients compared the ACAF Klotski technique with anterior cervical corpectomy and fusion (ACCF), revealing fewer complications and better postoperative outcomes for those treated with the ACAF technique.
- Results showed the ACAF group had a lower rate of neurological deterioration and intraoperative cerebrospinal fluid leakage compared to the ACCF group, making it a favorable option for en bloc type OPLL-DO treatment.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!