The medaka, Oryzias latipes, like other fish, have two distinct aromatase genes, the ovarian (cyp19a1) and brain (cyp19a2) forms. We previously reported that Ad4BP/SF-1, a member of the NR5A subfamily, plays an important role in the regulation of cyp19a1 expression in medaka ovarian follicles during vitellogenesis. In the present study, we investigated whether liver receptor homologue-1 (LRH-1), another NR5A subfamily member, is involved in the regulation of cyp19a2 expression in the medaka brain. In situ hybridization analysis revealed that LRH-1 was expressed in the hypothalamus, where it colocalized with aromatase (cyp19a2). We then showed by transient transfection assays that LRH-1 was able to increase expression of a cyp19a2 reporter gene in various mammalian cell lines, and that mutation of a putative LRH-1 binding site within the cyp19a2 promoter abolished this effect. Taken together, these findings suggest that LRH-1 plays a role in regulating cyp19a2 expression in the medaka brain. This is the first to demonstrate in vitro the activation of brain aromatase by LRH-1 in the vertebrate brain.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/mrd.20497 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sunlight-mediated environmental risks of tinidazole in seawater: A neglected ocular toxicity of photolysis mixtures.
J Hazard Mater
January 2025
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China; State Key Laboratory of Mariculture Breeding, Xiamen University, Xiamen 361102, China. Electronic address:
Tinidazole (TNZ), a common nitroimidazole antibiotic, is pervasive in aquatic ecosystems, posing potential threats to marine organisms. The environmental fate of TNZ, particularly under solar irradiation, and the associated secondary risks are not well characterized. Herein, the photochemical reactivity of TNZ and four other typical nitroimidazoles (i.
View Article and Find Full Text PDFFunctional test of a naturally occurred tumor modifier gene provides insights to melanoma development.
G3 (Bethesda)
January 2025
Xiphophorus Genetic Stock Center, Texas State University, San Marcos, TX 78666, USA.
Occurrence of degenerative interactions is thought to serve as a mechanism underlying hybrid unfitness in most animal systems. However, the molecular mechanisms underpinning the genetic interaction and how they contribute to overall hybrid incompatibilities are limited to only a handful of examples. A vertebrate model organism, Xiphophorus, is used to study hybrid dysfunction, and it has been shown from this model that diseases, such as melanoma, can occur in certain interspecies hybrids.
View Article and Find Full Text PDFAlthough sex determination is a fundamental process in vertebrate development, it is very plastic. Diverse genes became major sex determinants in teleost fishes. Deciphering how individual sex-determining genes orchestrate sex determination can reveal new actors in sexual development.
View Article and Find Full Text PDFToxicity of nickel, copper, and selenium in medaka embryos (oryzias latipes): a comparative study.
J Toxicol Sci
January 2025
Department of Creative engineering, National Institute of Technology, Ariake College.
Article Synopsis
- The study emphasizes the significance of nickel, copper, and selenium as essential biometals in various industries, but highlights their harmful impact as environmental pollutants when they exceed normal levels, particularly in aquatic ecosystems.
- Research on medaka fish embryos revealed that exposure to these biometals led to significant developmental abnormalities and increased mortality rates throughout a 14-day observation period.
- Gene expression analysis showed that copper was the most toxic, affecting over 4,700 genes, while all three biometals caused detrimental effects on fish embryo development, underscoring the necessity of monitoring biometal levels to protect aquatic life.
Temperature-dependent toxicity and mechanisms of florfenicol on the embryonic development of marine medaka (Oryzias melastigma).
Ecotoxicol Environ Saf
January 2025
Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou 515063, China; Guangdong Engineering Technology Research Center of Offshore Environmental Pollution Control, Shantou University, Shantou 515063, China. Electronic address:
The extensive use of antibiotics and their persistence in the environment have seriously threatened marine ecosystems in recent years. The frequent occurrence of extreme weather due to climate change has also increased the uncertainty of effective toxicity identification and risk assessment of the chemicals of concern. This study aimed to investigate the toxic effects and potential mechanisms of florfenicol (0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!