The separation of Staphylococcus epidermidis and Rhodococcus erythropolis bacteria was achieved with the use of Micro-Thermal Focusing Field-Flow Fractionation. This is the first performance of separation exploiting the Ludwig-Soret effect (thermal diffusion) of living biological cells, combined with lift forces and resulting in the focusing mechanism of separation. The experiments were carried out under carefully chosen experimental conditions preventing the denaturation of the bacteria.
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Separation of bacteria in temperature gradient: micro-thermal focusing field-flow fractionation.
J Biochem Biophys Methods
June 2007
Tomas Bata University, Faculty of Technology, TGM 275, 762 72 Zlín, Czech Republic.
The separation of Staphylococcus epidermidis and Rhodococcus erythropolis bacteria was achieved with the use of Micro-Thermal Focusing Field-Flow Fractionation. This is the first performance of separation exploiting the Ludwig-Soret effect (thermal diffusion) of living biological cells, combined with lift forces and resulting in the focusing mechanism of separation. The experiments were carried out under carefully chosen experimental conditions preventing the denaturation of the bacteria.
View Article and Find Full Text PDFMicro-thermal field-flow fractionation of bacteria.
J Chromatogr B Analyt Technol Biomed Life Sci
June 2007
Université de La Rochelle, Pôle Sciences et Technologie, Avenue Michel Crépeau, 17042 La Rochelle, France.
The retention of Staphylococcus epidermidis bacteria cells, achieved with the use of micro-thermal field-flow fractionation and described in this paper, represents the first experimental proof that the separation and characterization of the bio-macromolecules and biological particles is possible by exploiting Ludwig-Soret effect of thermal diffusion. The experiments were carried out under gentle experimental conditions preventing the denaturation of the bacteria. Lift forces, appearing at high linear velocities of the carrier liquid, generated the focusing mechanism of the retention which resulted in high-speed and high-performance separation performed in less than 10 min.
View Article and Find Full Text PDFEffect of channel width on the retention of colloidal particles in polarization, steric, and focusing micro-thermal field-flow fractionation.
J Chromatogr A
August 2004
Université de La Rochelle, Pôle Sciences et Technologie, Avenue Michel Crépeau, 17042 La Rochelle Cedex 01, France.
The effect of the channel width on the performance of separation by micro-thermal field-flow fractionation (micro-TFFF) of the carboxylated polystyrene latex particles was studied by using the particles in diameter range from 100 nm to 3800 nm. It has been shown that the retention order follows the anticipated polarization, steric, and focusing mechanism in the corresponding size range and under the specific conditions, appropriate to each channel thickness. However, the attractive interactions of the particles with the accumulation wall can complicate the separation as has been proven by the experiments carried out by using the carrier liquids of different ionic strengths.
View Article and Find Full Text PDFMicro-thermal focusing field-flow fractionation.
J Chromatogr B Analyt Technol Biomed Life Sci
February 2004
Pôle Sciences et Technologie, Université de La Rochelle, Avenue Michel Crépeau, 17042 La Rochelle Cedex 01, France.
Focusing mechanism was effectively exploited to separate large (micrometer-size) particles by using new micro-thermal field-flow fractionation (micro-TFFF). It has been shown that the retention order of micrometer-size particles at high field strength can be explained by the mechanism of steric exclusion only at lowest flow rates of the carrier liquid. A simplistic, purely mechanical model of steric exclusion is not accurate to describe the retention at higher flow rates where the focusing phenomenon appears.
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