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Proteomic analysis of exfoliation deposits. | LitMetric

Proteomic analysis of exfoliation deposits.

Invest Ophthalmol Vis Sci

Department of Ophthalmology, New York Eye and Ear Infirmary, New York, NY, USA.

Published: April 2007

AI Article Synopsis

  • * Researchers analyzed lens capsules from patients with and without exfoliation syndrome (XFS), identifying unique proteins linked to XFS through various biochemical methods and mass spectrometry.
  • * Key findings revealed not only known proteins like fibrillin-1 and fibronectin but also newly identified components such as clusterin and TIMP-3, highlighting the complexity of XFS and suggesting new research pathways into its development and progression.

Article Abstract

Purpose: To increase knowledge of the biochemical composition of lenticular exfoliation material (XFM) by using proteomic approaches.

Methods: Anterior lens capsules from patients with and without exfoliation syndrome (XFS) were homogenized in formic acid and subjected to cyanogen bromide (CNBr) cleavage, and the pattern of chemically generated fragments was compared by SDS-PAGE after silver staining. Unique XFS bands not present in control cases were excised, digested with TPCK-trypsin, and the resultant peptides sequenced with quadrupole time-of-flight mass spectrometry (MS). In parallel experiments, CNBr-fragmented XFM was separately digested in solution with trypsin and elastase, and the resultant peptide mixture was analyzed by liquid chromatography coupled to tandem MS followed by identification through homology searches at nonredundant protein databases. Immunolocalization of the MS-identified components were performed in XFS versus control samples by using conventional immunohistochemical methods and light microscopy.

Results: In addition to fibrillin-1, fibronectin, vitronectin, laminin, and amyloid P-component, which are well-known extracellular matrix and basement membrane components of XFM, the proteomic approaches identified the multifunctional protein clusterin and tissue inhibitor of metalloprotease (TIMP)-3 as well as novel molecules, among them fibulin-2, desmocollin-2, the glycosaminoglycans syndecan-3, and versican, membrane metalloproteases of the ADAM family (a disintegrin and metalloprotease), and the initiation component of the classic complement activation pathway C1q. In all cases, classic immunohistochemistry confirmed their location in XFM.

Conclusions: A novel solubilization strategy combined with sensitive proteomic analysis emphasizes the complexity of the XFS deposits and opens new avenues to study the molecular mechanisms involved in the pathogenesis and progression of XFS.

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Source
http://dx.doi.org/10.1167/iovs.06-0411DOI Listing

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