The 32-nm-diameter spherical viral particles found in the cytoplasm of Leishmania guyanensis CUMC1-1A sediment at 130S and have a buoyant density of approximately 1.4 g/ml in cesium chloride gradients. These particles contain a 5.3-kb double-stranded RNA, while single-stranded RNA that corresponds to the viral positive strand is associated with less-dense particles. These results suggest a conservative and sequential mode of LRV1 viral RNA replication that is exemplified by the ScV L-A virus of yeast.
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Transcriptomics analysis highlights potential ways in human pathogenesis in Leishmania braziliensis infected with the viral endosymbiont LRV1.
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Laboratório de Imunologia Celular Aplicada à Saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, Porto Velho, Brazil.
The parasite Leishmania (Viannia) braziliensis is widely distributed in Brazil and is one of the main species associated with human cases of different forms of tegumentary leishmaniasis (TL) such as cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML). The mechanisms underlying the pathogenesis of TL are still not fully understood, but it is known that factors related to the host and the parasite act in a synergistic and relevant way to direct the response to the infection. In the host, macrophages have a central connection with the parasite and play a fundamental role in the defense of the organism due to their ability to destroy intracellular parasites and present antigens.
View Article and Find Full Text PDFExploring Host-Specificity: Untangling the Relationship between () Species and Its Endosymbiont 1.
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September 2023
Leishmaniasis Research Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040360, Brazil.
A relevant aspect in the epidemiology of Tegumentary Leishmaniasis (TL) are the parasites carrying a viral endosymbiont, 1 (LRV1), a dsRNA virus. parasites carrying LRV1 are prone to causing more severe TL symptoms, increasing the likelihood of unfavorable clinical outcomes. LRV1 has been observed in the cultured strains of five () species, and host specificity was suggested when studying the LRV1 from and strains.
View Article and Find Full Text PDFRaising the suspicion of a non-autochthonous infection: identification of Leishmania guyanensis from Costa Rica exhibits a Leishmaniavirus related to Brazilian north-east and French Guiana viral genotypes.
Mem Inst Oswaldo Cruz
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Fiocruz-Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Pesquisa em Leishmanioses, Rio de Janeiro, RJ, Brasil.
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Virion structure of Leishmania RNA virus 1.
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Central European Institute of Technology, Masaryk University, Kamenice 753/5, Brno, 625 00, Czech Republic. Electronic address:
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Article Synopsis
- The lymphatic system is essential for the immune response against intracellular pathogens but also aids cancer cell spread, and its role in mucocutaneous leishmaniasis (MCL) has not been fully explored.
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- Researchers used various methods to verify that parasites can move through migrating immune cells and lymphatic systems, leading to new infections in distant areas, highlighting the complex pathways of parasite dissemination.
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