AI Article Synopsis
- Two genes similar to glycerol-3-phosphate dehydrogenases were isolated from the yeast Pichia jadinii using PCR amplification and genomic libraries.
- DNA sequencing of the full-length genes PjGPD1 and PjGPD2 revealed open reading frames of 1182 bp and 1185 bp, respectively.
- A complementation study showed that PjGPD1 can restore growth in a Saccharomyces cerevisiae mutant under osmotic stress, while PjGPD2 has temperature-sensitive complementation effects.
Article Abstract
Two genes coding for proteins with a high degree of sequence similarity to glycerol-3-phosphate dehydrogenases have been isolated from the yeast Pichiajadinii. Fragments of the genes were PCR-amplified with degenerated primers from genomic DNA of P. jadinii. Clones containing the full-length genes PjGPDI and PjGPD2 were isolated by screening genomic libraries. DNA sequencing revealed open reading frames (ORFs) of 1182 bp and 1185 bp for PjGpdlp and PjGpd2p, respectively. In a complementation study PjGPD1 rescued the growth defect of a Saccharomyces cerevisiae Agpdl mutant strain under osmotic stress, while complementation by PjGPD2 is temperature sensitive. The sequences of the PjGPD1 and PjGPD2 ORFs have been submitted to the EMBL Nucleotide Sequence Database under Accession No. AJ632339 and AJ632340, the sequences of the corresponding genomic DNA fragments under Accession No. AJ632341 and AJ635370, respectively.
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| http://dx.doi.org/10.1080/10425170600807165 | DOI Listing |
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