Nicotinamide adenine dinucleotide synthetase (NadE) is an essential enzyme for bacterial pathogens and is thus a promising antibacterial target. It catalyzes the conversion of nicotinic acid adenine dinucleotide to nicotinamide adenine dinucleotide. Changes in chemical shifts that occur in the nicotinic acid ring as it is converted to nicotinamide can be used for monitoring the reaction. A robust nuclear magnetic resonance-based activity assay was developed using robotically controlled reaction initiation and quenching. The single-enzyme assay has less potential for false positives compared to a coupled activity assay and is especially well suited to the high concentration of compounds in fragment screens. The assay has been used to screen fragment libraries for NadE inhibitors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1177/1087057107299717 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transcriptomic and Metabolomic Insights Into the Prebiotic Potential of Camellia Seed Oil for Enhancing Proliferation In Vitro.
Food Sci Nutr
January 2025
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering Guizhou University Guiyang China.
Camellia seed oil (CSO), a potential prebiotic agent, can significantly increase the relative abundance of () in mice gut microbiota following oral administration, this study aims to investigate the enhancing effect in vitro. The results showed that after 24-h co-cultivation with 0.5% (v/v) CSO, the growth of increased from 11.
View Article and Find Full Text PDFSuccinic Acid Production With -Influence of an Electric Potential on the Intercellular NADH/NAD Balance.
Eng Life Sci
January 2025
Mechanical and Process Engineering RPTU Kaiserslautern-Landau Kaiserslautern Germany.
Bioelectrochemical systems (BESs) offer a sustainable method for chemical production, including the enhanced production of succinic acid. By combining fermentation with BES, it could be possible to achieve sustainable succinic acid production and CO fixation using . In literature, the potential application of BES is commonly associated with increased succinate yields, as it is expected to enhance the availability of NADH, thereby influencing the intracellular nicotinamide adenine dinucleotide (NADH/NAD) balance.
View Article and Find Full Text PDFObjectives: The current experiment was conducted to investigate the combined effect of levofloxacin (LVX) and metformin treatment on blood glucose levels, malondialdehyde (MDA),nitrite levels, and anxiety in streptozotocin (STZ)+ nicotine adenine dinucleotide (NAD)-induced diabetic rats.
Materials And Methods: In this study, Wistar rats have been used. After receiving a single dose of STZ + NAD (45 mg/kg, + 50 mg/kg, ), the rats developed diabetes.
4-Pyridone-3-carboxamide-1-β-D-ribonucleoside Reduces Cyclophosphamide Effects and Induces Endothelial Inflammation in Murine Breast Cancer Model.
Int J Mol Sci
December 2024
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdańsk, Poland.
4-pyridone-3-carboxamide-1-β-D-ribonucleoside (4PYR) is a nicotinamide derivative, considered a new oncometabolite. 4PYR formation induced a cytotoxic effect on the endothelium. Elevated blood 4PYR concentration was observed in patients with cancer.
View Article and Find Full Text PDFGasoline exhaust particles induce MMP1 expression via Nox4-derived ROS-ATF3-linked pathway in human umbilical vein endothelial cells.
Toxicology
January 2025
Division of Cardiovascular Disease Research, Department of Chronic Disease Convergence Research, Korea National Institute of Health, Cheongju, Republic of Korea.
Gasoline exhaust particles (GEP) are risk factors for cardiovascular disease. Activating transcription factor 3 (ATF3) is a transcription factor known to form a heterodimer with AP-1 transcription factors for its target gene expression. However, the involvement of ATF3 in GEP-induced gene expression in human umbilical vein endothelial cells (HUVECs) has not been investigated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!