A total of 100 cases of burn were examined and screened bacteriologically for evidence of infection by surface swab culture, quantitative full thickness punch biopsy culture and blood culture. Gram negative organisms predominates the gram positive ones. Surface swab was found to correlate well with the biopsy as far the identification of causative organism is concerned. However, the latter technique was found to be more valuable as it also gives the critical load (>10(5)CFU/g of tissue) of the organism beyond which metastatic invasion of the organism takes place (p<0.01), thus obviating the repeated need for blood culture in burn patients.
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BMJ Case Rep
January 2025
Dermatology, Ninewells Hospital and Medical School Department of Dermatology, Dundee, UK.
A man in his early 40s presented with extensive skin lesions in the groin and genital regions. The lesions were erythematous and maculopapular but evolved into vesicles and pustules with surrounding cellulitis. He stated that he grips livestock between his legs for sheep shearing.
View Article and Find Full Text PDFAm J Infect Control
January 2025
Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal. Electronic address:
Background: Twelve bacterial families were identified as global priority pathogens by the World Health Organization in 2017, recognizing the greatest threat they pose to human health and the declining antibiotic efficacy. Robotics has emerged as a swift and contactless tool for disinfecting bacterial surface contamination in healthcare facilities, however, head-to-head comparison of disinfection efficacy of robotic versus manual disinfections is limited. This study aimed at comparing how robotic disinfection performs over manual disinfection against the global priority pathogens in the healthcare setting.
View Article and Find Full Text PDFAnal Chem
January 2025
CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China.
Droplet microfluidics is a powerful method for digital droplet polymerase chain reaction (ddPCR) applications. However, precise droplet control, bulky peripherals, and multistep operation usually required in droplet detection process hinder the broad application of ddPCR. Here, a contracted channel droplet reinjection chip is presented, where droplets can be self-separated and detected one by one at intervals.
View Article and Find Full Text PDFFront Public Health
January 2025
Department of Environmental Health, College of Medicine and Health Sciences, Debre Markos University, Debre Markos, Ethiopia.
Background: Meat is a good source of protein in the human diet, and more than three-quarters of the world's population consumes it. It is the most perishable food item since it has enough nutrients to enable microbial growth. In underdeveloped nations, animals are routinely slaughtered and sold in unsanitary conditions, compromising the bacteriological quality and safety of the meat received from the animals.
View Article and Find Full Text PDFFungal Syst Evol
December 2024
Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands.
Novel species of fungi described in this study include those from various countries as follows: , from accumulated snow sediment sample. , on leaf spots of . , on submerged decaying wood in sea water, on , as endophyte from healthy leaves of .
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