AI Article Synopsis
- RT-PCR was used to clone glutathione transferase Pi cDNAs from three fish species: Cyprinus carpio, Hypophthalmichthys molitrix, and Carassius auratus, which showed an open reading frame (ORF) of 627 bp encoding 208 amino acids.
- The sequence similarity analysis indicated that fish share 50% similarity with mammals, 33% with amphibians, and 15% with arthropods, with a high average similarity (about 85%) among the four cyprinid species.
- A phylogenetic tree created from GST Pi amino acid sequences identified two major clusters, one for mammals and another for fish, and suggested that freshwater fish might have a stronger detox
Article Abstract
Using RT-PCR method, the glutathione transferase Pi cDNAs were cloned from Cyprinus carpio, Hypophthalmichthys molitrix, and Carassius auratus. The open reading frames (ORFs) from the 3 fishes were 627 bp long (encoding for 208 amino acids) with the initial code ATG and the terminal code TGA. The sequence similarity was 50% between fish and mammals, 33% between fish and amphibian, and 15% between fish and arthropoda, respectively. The sequence similarity was big among fishes, and the average value of the 4 cyprinids was about 85%. Phylogenetic tree was constructed for 13 species based on GST Pi amino acid sequences using MP (Maximum Parsimony) method. Two major clusters were recognized: cluster one consisted of Mammals (bootstrap 100) and cluster two consisted of fishes (bootstrap 93). Based on the sequences analyses of N/C domain of GST Pi, we proposed the detoxification mechanism of freshwater fishes that were thought to have stronger tolerance to microcystins.
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| http://dx.doi.org/10.1360/yc-007-0349 | DOI Listing |
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