Capillary electrophoresis in 770 nanometer inner diameter capillaries coupled to electrochemical detection with an etched electrode matching an etched capillary (etched electrochemical detection) has been used with ultrasmall sampling to inject subcellular samples from intact single mammalian cells. Separations of cytoplasmic samples taken from rat pheochromocytoma cells have been achieved. As little as 8% of the total volume of a single cell has been sampled and analyzed. Dopamine has been identified and quantified in these PC12 cells using this technique. The average cytoplasmic level of dopamine in rat pheochromocytoma cells has been determined to be 240 ± 60 μM. The use of electrophoresis in 770 nanometer inner diameter capillaries with electrochemical detection to monitor cytoplasmic neurotransmitters at the single cell level can provide information about complex cellular functions such as neurotransmitter storage and synthesis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1821352 | PMC |
| http://dx.doi.org/10.1002/elan.200403240 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Highly stable 3D cellulose micro-rolls support TiO for efficient photocatalysis degradation experiment under weak light conditions.
Int J Biol Macromol
January 2025
College of Chemistry and Materials Engineering, Zhejiang A&F University, Hangzhou, Zhejiang Province 311300, PR China. Electronic address:
Immobilization of nanometer-scale photocatalysts on a 3D polymeric substrate could play several complementary roles in photocatalysis, such as providing mechanical stability, facilitating easy recycling after usage, enhancing adsorption capability, and improving light harvesting properties through multiple reflections. To achieve stable and efficient photocatalysis under weak light conditions, 3D cellulose micro-rolls were introduced into the photocatalytic composites. Here, the formation of micro-rolls is attributed to the presence of titania nanoparticles, which generate uneven shrinkage stress in cellulose during the freeze-drying process, thereby inducing the cellulose to curl up.
View Article and Find Full Text PDFImproving the Cellular Internalization of Zr(IV) Nanocages by Tuning Hydrophilicity and Lipophilicity.
Angew Chem Int Ed Engl
October 2024
State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002, PR China.
Nanoscale molecular materials have emerged as a new class of compounds at the nanometer scale with well-defined chemical structures, remarkable uniformity and high reproducibility. Among these materials, zirconium-based metal-organic cages (MOCs) have attracted significant attention due to their exceptional stability and applications in catalysis, recognition and separation and so on. However, their poor water solubility impedes their biomedical applications.
View Article and Find Full Text PDFUnderstanding the chemistry of mesostructured porous nanoreactors.
Nat Rev Chem
December 2024
College of Energy Materials and Chemistry, School of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, China.
Porous nanoreactors mimic the structures and functions of cells, providing an adaptable material with multiple functions and effects. These reactors can be nanoscale containers and shuttles or catalytic centres, drawing in reactants for cascading reactions with multishelled designs. The detailed construction of multi-level reactors at the nanometre scale remains a great challenge, but to regulate the reaction pathways within a reactor, designs of great intricacy are required.
View Article and Find Full Text PDFThe epsilon toxin from Clostridium perfringens stimulates calcium-activated chloride channels, generating extracellular vesicles in Xenopus oocytes.
Pharmacol Res Perspect
October 2024
Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences-Campus Bellvitge, University of Barcelona, Barcelona, Spain.
The epsilon toxin (Etx) from Clostridium perfringens has been identified as a potential trigger of multiple sclerosis, functioning as a pore-forming toxin that selectively targets cells expressing the plasma membrane (PM) myelin and lymphocyte protein (MAL). Previously, we observed that Etx induces the release of intracellular ATP in sensitive cell lines. Here, we aimed to re-examine the mechanism of action of the toxin and investigate the connection between pore formation and ATP release.
View Article and Find Full Text PDFSynchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale.
Sci Rep
July 2024
Department of Chemistry, Química-Física Molecular, University of Coimbra, 3004-535, Coimbra, Portugal.
Article Synopsis
- The study evaluated how human osteosarcoma cells respond to the chemotherapy drug cisplatin using advanced nanospectroscopy techniques at a specialized research facility in the UK.
- The method used, synchrotron-based Fourier Transform InfraRed nanospectroscopy, allowed researchers to analyze the cells at a very small scale (hundreds of nanometers), providing detailed information about both the structure and biochemical changes occurring in the cells.
- Results indicated that cisplatin significantly impacted proteins and cellular lipids, with a notable concentration of these effects occurring in the cytoplasm, marking a pioneering application of this nanospectroscopy technique in cancer research.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!