Two isolates of Acanthamoeba sp. CJY/SI and CJY/S2 were received from soil in Yanji of Jilin Province. Full 18S rDNA gene was amplified using PCR, cloned and sequenced. The results were analyzed by software Clustal X. The full length of CJY/S1 and CJY/S2 is 2255 bp and 2252 bp respectively, both belong to T4 genotype.
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