In the context of the process of revising EU Directive 86/609/EEC on the protection of animals used in experiments, the European Commission conducted a public internet consultation for EU citizens in mid-2006. Simultaneously, the Commission requested opinions from experts on specific animal welfare issues. The results of both consultations were published in the internet in December 2006. An overwhelming majority of EU citizens answered that the protection of laboratory animals currently is poor and that efforts are needed to improve the level of welfare for these animals. Additionally, they request increased transparency and public participation in the determination when and how the use of animals in experiments is to be considered acceptable. They also asked for an increased promotion of the research for replacing animal experiments. Amongst other issues, the experts called for an extension of the scope of the Directive to also cover animals used in basic research and the establishment of a compulsory authorization procedure which should include a concrete ethical review process. The estimations put forward on the consequences of introducing a retrospective analysis of projects with animal experiments were controversial just as the opinions submitted regarding different options regarding a ban to using wild caught primates and their direct offspring. All in all, both the responses of the citizens and experts consultations are a promising basis to justify the need to improve the protection of animals used in experiments within the EU.
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http://dx.doi.org/10.14573/altex.2007.1.41 | DOI Listing |
Neurotox Res
January 2025
Molecular Neuropsychiatry Section, Intramural Research Program, NIH/ NIDA, 21224, Baltimore, MD, U.S.A.
To identify factors involved in methamphetamine (METH) neurotoxicity, we comprehensively searched for genes which were differentially expressed in mouse striatum after METH administration using differential display (DD) reverse transcription-PCR method and sequent single-strand conformation polymorphism analysis, and found two DD cDNA fragments later identified as mRNA of Nedd4 (neural precursor cell expressed developmentally downregulated 4) WW domain-binding protein 5 (N4WBP5), later named Nedd4 family-interacting protein 1 (Ndfip1). It is an adaptor protein for the binding between Nedd4 of ubiquitin ligase (E3) and target substrate protein for ubiquitination. Northern blot analysis confirmed drastic increases in Ndfip1 mRNA in the striatum after METH injections, and in situ hybridization histochemistry showed that the mRNA expression was increased in the hippocampus and cerebellum at 2 h-2 days, in the cerebral cortex and striatum at 18 h-2 days after single METH administration.
View Article and Find Full Text PDFIn Vitro Cell Dev Biol Anim
January 2025
College of Traditional Chinese Medicine, Xinjiang Uygur Autonomous Region, Xinjiang Medical University, Urumqi, 830063, China.
The aim of this study is to assess the impact of Tianxiangdan (TXD) on lipophagy in foam cells and its underlying mechanism in treating atherosclerosis, particularly focusing on its efficacy in lowering blood lipids. In vivo, ApoE-/- atherosclerosis mouse models were established for group intervention. Blood lipid levels of the mice were measured, lipid deposition and autophagy levels in atherosclerotic plaques were assessed, and co-localization of lipid droplets and autophagosomes was examined.
View Article and Find Full Text PDFMetab Brain Dis
January 2025
Key Laboratory of Prevention and treatment of cardiovascular and cerebrovascular diseases of Ministry of Education, Gannan Medical University, Ganzhou, 341000, China.
Cerebral ischemia-induced pyroptosis contributes to the dissemination of neuroinflammation, and Nod-like receptor protein-3 (NLRP3) inflammasome plays a key role in this process. Previous studies have indicated that Genistein-3'-sodiumsulfonate (GSS) can inhibit neuroinflammation caused by cerebral ischemia, exert cerebroprotective effects, but its specific mechanism has not been comprehensively understood. The aim of this study was to explore the effect of GSS on ischemic stroke-induced cell pyroptosis.
View Article and Find Full Text PDFLasers Med Sci
January 2025
Hangzhou Third People's Hospital, Hangzhou, China.
Background: Post-inflammatory hyperpigmentation (PIH) is a common cosmetic concern, often leading to significant psychological distress for the patients. With the widespread application of lasers including ablative fractional resurfacing (AFR) with a 10,600 nm CO laser, PIH caused by lasers is becoming increasingly common. But due to the absence of an appropriate animal research model, our understanding of pathophysiological mechanisms and preventive strategies for PIH remains limited.
View Article and Find Full Text PDFFish Physiol Biochem
January 2025
São Paulo State University (UNESP), Aquaculture Center of UNESP, Jaboticabal, Sao Paulo, Brazil.
This study examined the energy-dependent physiological responses, including stress, innate immune, and antioxidant systems, as well as indicators of energy mobilization, in pacu (Piaractus mesopotamicus) exposed to intermittent cold, aiming to assess the correlations between these responses. The fish were acclimated to 28 °C, divided into two groups, a control group maintained at 28 °C, and another exposed to 16 °C for two 24 h periods with a 5-day interval between them. The fish were sampled at six time points: baseline (after acclimatization to 28 °C), 24 h after the 1st exposure to 16 °C, after 5 days of recovery at 28 °C, 24 h after the 2nd exposure to 16 °C, and after 24 and 48 h of recovery at 28 °C.
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