AI Article Synopsis
- - The study focuses on the role of CD4+CD25+ regulatory T cells in the immunosuppression of gastric cancer patients and aims to understand their cytokine secretion mechanisms, particularly examining IFN-gamma, IL-4, IL-10, and TGF-beta.
- - Researchers isolated different T cell populations from the blood of gastric cancer patients and used advanced techniques to measure cytokine production, finding higher levels of suppressive cytokines (IL-10 and TGF-beta) from regulatory T cells compared to non-regulatory T cells.
- - Results indicate that the increased presence of CD4+CD25+ T cells in gastric cancer patients is linked to immune suppression, primarily through the production of TGF-beta
Article Abstract
Background & Objective: CD4+CD25+ regulatory T cells play a crucial role in the immunosuppression of gastric cancer patients, but the mechanism is still unknown. This study was to investigate the secretion of intracellular and extracellular cytokines interferon-gamma (IFN-gamma), interleukin-4 (IL-4), IL-10 and tumor growth factor-beta (TGF-beta) from CD4+CD25+ regulatory T cells in gastric cancer patients, and evaluate their roles in the immunosuppression of gastric cancer.
Methods: Peripheral blood mononuclear lymphocytes of gastric cancer patients were prepared routinely. CD4+CD25+ T cells and CD4+CD25- T cells were isolated by magnetic activated cell sorting (MACS) method, and identified by flow cytometry. The cytokine secretion of CD4+CD25+ T cells and CD4+CD25- T cells was detected by intracellular analysis of cytokine production (IFN-gamma, IL-4 and IL-10) and ELISA (IFN-gamma, IL-10 and TGF-beta).
Results: The proportion of CD4+CD25+ T cells to CD4+ T cells was significantly higher in gastric cancer patients than in healthy controls (P<0.05). After 96-hour cell culture, no matter in gastric cancer patients or in healthy controls, the secretion of IL-10 and TGF-beta were significantly higher from CD4+CD25+ T cells than from CD4+CD25- T cells (P<0.05), but the secretion of IFN-gamma was significantly lower from CD4+CD25+ T cells than from CD4+CD25- T cells (P<0.05).
Conclusion: The immunosuppression of CD4+CD25+ regulatory T cells in gastric cancer may relate to suppressive cytokines, especially TGF-beta.
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