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Human fallopian tube organoids provide a favourable environment for sperm motility.
Hum Reprod
January 2025
IRSD, Université de Toulouse, INSERM, INRAE, ENVT, Univ Toulouse III-Paul Sabatier (UPS), Toulouse, France.
Study Question: Does a human fallopian tube (HFT) organoid model offer a favourable apical environment for human sperm survival and motility?
Summary Answer: After differentiation, the apical compartment of a new HFT organoid model provides a favourable environment for sperm motility, which is better than commercial media.
What Is Known Already: HFTs are the site of major events that are crucial for achieving an ongoing pregnancy, such as gamete survival and competence, fertilization steps, and preimplantation embryo development. In order to better understand the tubal physiology and tubal factors involved in these reproductive functions, and to improve still suboptimal in vitro conditions for gamete preparation and embryo culture during IVF, we sought to develop an HFT organoid model from isolated adult stem cells to allow spermatozoa co-culture in the apical compartment.
Ipsilateral ectopic pregnancy in the distal remaining part of the previously removed Fallopian tube: a case report.
J Med Case Rep
December 2024
Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Background: Ectopic pregnancy, occurring outside the uterine cavity, poses a significant health risk, with Fallopian tube involvement being predominant. Recurrent ectopic pregnancy, particularly in the ipsilateral remnant of a previously removed tube, is a rare and poorly understood phenomenon. Here, we present a case of recurrent ectopic pregnancy occurring in the distal remnant of the right fallopian tube following ipsilateral incomplete salpingectomy in a 22-year-old woman.
View Article and Find Full Text PDFComparative performance analysis of different microfilaria testing methods for Dirofilaria immitis in canine blood.
Parasit Vectors
November 2024
Department of Veterinary Pathobiology, Oklahoma State University College of Veterinary Medicine, Stillwater, OK, USA.
Article Synopsis
- Microfilaria (MF) testing is crucial for diagnosing canine heartworm, and the American Heartworm Society recommends annual testing for all dogs, regardless of their prevention history.
- Various MF testing methods exist, each differing in reliability, cost, and complexity; the study evaluates methods like the modified Knott test (MKT) and PCR, which are the most reliable but also more expensive and time-consuming.
- While the most reliable tests can identify heartworm even at low levels and confirm species, simpler tests like thin smears are less reliable, especially for accurate detection of MF-positive dogs at lower burdens.
A rare case of endotracheal tube cuff leakage with no detectable decrease in cuff pressure.
JA Clin Rep
November 2024
Department of Anesthesiology, Wakayama Medical University School of Medicine, Wakayama, 641-8510, Japan.
Background: Common causes of air leakage around an endotracheal tube include insufficient endotracheal tube cuff inflation and damage to the cuff, while damage to the pilot balloon or pilot balloon tubing is relatively rare.
Case Presentation: A 74-year-old female with vertebral osteomyelitis was scheduled for an extreme lateral interbody fusion followed posterior fixation. A fiber-optic light was utilized as part of the surgical illuminator.
Employing a switching valve to automate external standard calibration in inductively coupled plasma optical emission spectrometry.
Talanta
December 2024
Department of Chemistry, Wake Forest University, Salem Hall, Box 7486, Winston-Salem, NC, 27109, United States. Electronic address:
The traditional external standard calibration method (EC) is automated and simplified using a four-port switching valve (SV4) and a multi-signal approach that enables the generation of several calibration points from a single calibration solution. The SV4-EC method is applied to inductively coupled plasma optical emission spectrometry (ICP-OES) and is based on gradient dilution taking place within the instrument's sample introduction tubing. Both the calibration solution and the samples are diluted by a blank solution containing an internal standard species.
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