AI Article Synopsis
- The study examined the effects of baicalin, a compound from Scutellaria plants, on human leukemia HL-60 cells, focusing on cell viability and apoptosis.
- Baicalin was shown to induce cytotoxicity in a dose- and time-dependent manner by activating caspase-3 and increasing reactive oxygen species (ROS) and calcium levels while decreasing mitochondrial membrane potential.
- The research concluded that baicalin triggers apoptosis in HL-60 cells through various mechanisms, enhancing certain pro-apoptotic factors and reducing anti-apoptotic ones.
Article Abstract
Background: Chemotherapy agents, particularly those that can induce apoptosis, are the major intervening strategy in the treatment of leukemia. In this study, we investigated the effects of baicalin (a compound obtained from Scutellaria baicalensis Georgi and S. rivularis Benth Labiateae) on the viability, induction of apoptosis and associated mechanism in human leukemia HL-60 cells.
Materials And Methods: The cell viability and apoptosis was examined by flow cytometric analysis. The results showed that baicalin induced cytotoxicity in a dose- and time-dependent manner through the activation of caspase-3, as shown by treatment of HL-60 cells with an inhibitor of caspase-3 (z-VAD-fmk). Baicalin increased the levels of ROS, Ca2+ and decreased mitochondrial membrane potential in HL-60 cells. Western blot demonstrated that baicalin promoted the levels of Gadd153, Bax, cytochrome c and caspase-3 and -12, but decreased the levels of Grp78 and Bcl-2 in HL-60 cells.
Conclusion: Baicalin was found to induce apoptosis in HL-60 cells through multiple pathways.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Sodium hyaluronate microcapsules to promote antitumor selectivity of anacardic acid.
Int J Biol Macromol
January 2025
Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Campus of Pici, 60440-900 Fortaleza, CE, Brazil. Electronic address:
Anacardic acid (AA) is a phenolic lipid extracted from cashew nutshell liquid that has antitumor activity. Given the high hydrophobicity of this compound and aiming to create efficient vehicle for its administration in aqueous systems, the objective of the present work was to develop a microcapsule (MCAA) by spray dryer technique, based on the polysaccharide sodium hyaluronate (SH), containing AA as its core, encapsulated from nanoemulsion. The Encapsulation Efficiency of MCAA presented a value equal to 95.
View Article and Find Full Text PDFTetrabromobisphenol A, but not bisphenol A, disrupts plasma membrane homeostasis in myeloid cell models - A novel threat from an established persistent organic pollutant.
Sci Total Environ
January 2025
Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland; Laboratory of Transcriptional Regulation, Institute of Medical Biology PAS, Lodz, Poland. Electronic address:
Article Synopsis
- The study investigates the effects of Bisphenol A (BPA) and Tetrabromobisphenol A (TBBPA) on the dynamics of biological membranes, focusing on how these persistent organic pollutants impact myeloid cell lines.
- It was found that TBBPA specifically disrupts the plasma membrane's biophysical homeostasis, increasing mobility and decreasing order, while BPA showed no significant effects.
- The findings highlight TBBPA's potential to impair immune function, emphasizing the environmental toxicity concerns associated with persistent organic pollutants.
Naringenin, a Food Bioactive Compound, Reduces Oncostatin M Through Blockade of PI3K/Akt/NF-κB Signal Pathway in Neutrophil-like Differentiated HL-60 Cells.
Foods
January 2025
Center for Converging Humanities, Kyung Hee University, Seoul 02447, Republic of Korea.
Oncostatin M (OSM) plays a crucial role in diverse inflammatory reactions. Although the food bioactive compound naringenin (NAR) exerts various useful effects, including antitussive, anti-inflammatory, hepatoprotective, renoprotective, antiarthritic, antitumor, antioxidant, neuroprotective, antidepressant, antinociceptive, antiatherosclerotic, and antidiabetic effects, the modulatory mechanism of NAR on OSM expression in neutrophils has not been specifically reported. In the current work, we studied whether NAR modulates OSM release in neutrophil-like differentiated (d)HL-60 cells.
View Article and Find Full Text PDFNovel Copper(II) Coordination Compounds Containing Pyridine Derivatives of -Methoxyphenyl-Thiosemicarbazones with Selective Anticancer Activity.
Molecules
December 2024
Laboratory of Advanced Materials in Biopharmaceutics and Technics, Institute of Chemistry, Moldova State University, MD-2009 Chisinau, Moldova.
Ten coordination compounds, [Cu(L)Cl] (), [Cu(L)NO] (), [Cu(L)Cl] (C3), [Cu(L)NO] (), [Cu(L)Cl] (), [Cu(L)NO] (), [Cu(L)NO] (), [Cu(L)Cl] (), [Cu(L)Cl] (), and [Cu(L)NO] (), containing pyridine derivatives of -methoxyphenyl-thiosemicarbazones were synthesized and characterized. The molecular structure of four compounds was investigated using single crystal X-ray diffraction. Spectral analysis techniques such as FT-IR, H NMR, C NMR, elemental analysis, and molar conductivity were used for all the synthesized compounds.
View Article and Find Full Text PDFSynthetic Studies on Vitamin D Derivatives with Diverse but Selective Biological Activities.
Chem Pharm Bull (Tokyo)
January 2025
Faculty of Pharmaceutical Sciences, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
Article Synopsis
- A-ring modifications in 1α,25-dihydroxyvitamin D enhance its binding to the vitamin D receptor (VDR) and increase its stability in cells by resisting metabolism, leading to longer-lasting effects.
- Various modified A-ring precursors synthesized from d-glucose showed specific biological activities with minimal calcemic side effects, including MART-10's potent antitumor effects in cancer models and AH-1's superior bone-forming properties in osteoporosis models compared to natural vitamin D.
- Ongoing research includes developing a library of fluorinated vitamin D analogs with potential anti-inflammatory effects and therapeutic applications for conditions like psoriasis, alongside the creation of the VDR-silent analog KK-052, which selectively inhibits SREBP/SC
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!