Candida bombicola is a yeast species known to synthesize glycolipids. Although these glycolipids find several industrial, cosmetic and pharmaceutical applications, very little is known about the genetics of C. bombicola. A basic tool for genetic study and modification is the availability of an efficient transformation and selection system. In order to develop such a system, the URA3 gene of Candida bombicola was isolated using degenerate PCR and genomic walking. The gene encodes for an enzyme of 262 amino acids and shows high homology with the known orotidine-5'-phosphate decarboxylases of several other yeast species. The functionality of the gene was proved by complementation of a URA3-negative Saccharomyces cerevisiae strain.
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Article Synopsis
- Starmerella bombicola, a yeast strain, efficiently produces sophorolipids from oil refinery waste, specifically palm and soy fatty acid distillates, yielding higher amounts compared to vegetable oils.* -
- The produced sophorolipids demonstrate strong antimicrobial, anti-biofilm, and antifungal properties, effectively inhibiting the growth of various bacteria and fungi.* -
- This research highlights the potential to convert industrial waste into valuable biosurfactants with significant antimicrobial capabilities, offering an eco-friendly solution for waste utilization.*
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