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Efficient differentiation between Pullorum and Gallinarum by a -based PCR-HRM.
Avian Pathol
January 2025
Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225125, People's Republic of China.
Pullorum (. Pullorum) and Gallinarum (. Gallinarum) are the biovars of serovar Gallinarum that are responsible for pullorum disease and fowl typhoid in poultry, respectively.
View Article and Find Full Text PDFDeletion of pagL and arnT genes involved in LPS structure and charge modulation in the Salmonella genome confer reduced endotoxicity and retained efficient protection against wild-type Salmonella Gallinarum challenge in chicken.
Vet Res
January 2025
College of Veterinary Medicine, Jeonbuk National University, Iksan Campus, Iksan, 54596, Republic of Korea.
Fowl typhoid (FT) poses a significant threat to the poultry industry and can cause substantial economic losses, especially in developing regions. Caused by Salmonella Gallinarum (SG), vaccination can prevent FT. However, existing vaccines, like the SG9R strain, have limitations, including residual virulence and potential reversion of pathogenicity.
View Article and Find Full Text PDFIdentification of Salmonella enterica biovars Gallinarum and Pullorum and their antibiotic resistance pattern in integrated crop-livestock farms and poultry meats.
Access Microbiol
September 2024
Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.
Due to consumer demand, many conventional poultry farms are now growing poultry without antibiotics or synthetic chemicals. In addition to this, pasture/organic poultry farms have increased significantly in the USA, and they are also antibiotic- and chemical-free. According to recent reports, both antibiotic-free conventional and pasture poultry farmers are facing the re-emergence of bacterial diseases.
View Article and Find Full Text PDFConstruction, molecular characterization, and safety assessment of mutant of Gallinarum.
Front Microbiol
November 2024
Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
This study involves the development and molecular characterization of the isogenic markerless knockout mutant SG Δ, a genetically engineered live attenuated strain aimed at controlling Gallinarum (SG) infection in poultry. The mutant was generated by deleting the gene using -Red recombination technology, impairing adenylosuccinate lyase, necessary for purine biosynthesis. An 1,180 bp deletion was engineered within the gene, leaving a residual 298 bp genomic scar resulting in a purine auxotrophic mutant.
View Article and Find Full Text PDFBiochemical perturbations associated with Salmonella gallinarum infection in laying hens: Is oxidative stress implicated?
Acta Vet Hung
December 2024
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, 410002, Enugu State, Nigeria.
The aim of this study was to investigate some biochemical and tissue changes associated with Salmonella gallinarum infection in laying hens (LHs), and the complicities of oxidative stress (OS). Fifty LHs were assigned to two groups of 25 LHs infected with S. gallinarum (109 cfu*mL-1 of S.
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