AI Article Synopsis
- Cholinergic neuron degeneration is a key feature of Alzheimer's disease, prompting research into two radioligands for molecular imaging in a rat model.
- After inducing cholinergic degeneration, ex vivo autoradiography revealed a significant decrease in the uptake of (123)IBVM, indicating effective targeting of the vesicular acetylcholine transporter.
- Additionally, moderate reductions in (125)I-A-85380 binding in brain regions highlighted the potential of this marker for imaging nicotinic acetylcholine receptors, showcasing its unique advantages compared to other radioligands in similar studies.
Article Abstract
Degeneration of cholinergic neurons is a well known characteristic of Alzheimer's disease (AD). Two radioligands were studied in a rat model of cholinergic degeneration to evaluate their potential efficacy for molecular imaging of AD. Following specific cholinergic-cell immunolesioning with 192 IgG-saporin (SAP), ex vivo autoradiography was performed with (123)IBVM, a radioligand which targets the vesicular acetylcholine transporter (VAChT). Following the decay of (123)I, the same animals had in vitro autoradiography performed with (125)I-A-85380, a marker for nicotinic acetylcholine receptors (nAChRs). As expected significant, widespread decreases in (123)IBVM uptake were observed in SAP treated animals. Moderate but significant reductions in (125)I-A-85380 binding in the hippocampus (Hip) and cerebellum (Cbm) were also observed following SAP immunolesioning. The results with (123)IBVM confirm and extend previous work investigating the uptake of radioiodinated IBVM in this animal model. The results with (125)I-A-85380 are unique and are in contrast with work performed in this animal model with other nAChR radioligands, indicating the favourable properties of this radioligand for molecular imaging.
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| http://dx.doi.org/10.1016/j.neulet.2006.08.065 | DOI Listing |
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