Sample preparation for two-dimensional gel electrophoresis using pressure cycling technology.

Anal Biochem

Proteomics and Small Molecules Applications Laboratory, Pressure BioSciences, Woburn, MA 01801, USA.

Published: April 2007

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769960PMC
http://dx.doi.org/10.1016/j.ab.2007.01.033DOI Listing

Publication Analysis

Top Keywords

sample preparation
4
preparation two-dimensional
4
two-dimensional gel
4
gel electrophoresis
4
electrophoresis pressure
4
pressure cycling
4
cycling technology
4
sample
1
two-dimensional
1
gel
1

Similar Publications

Importance: An increasing number of older adults are undergoing surgery. Older adults face significant challenges throughout the spectrum of perioperative care. No frameworks exist to support primary care clinicians in helping older adults navigate perioperative care beyond preoperative medical clearance.

View Article and Find Full Text PDF

Automated High-Throughput Affinity Capture-Mass Spectrometry Platform with Data-Independent Acquisition.

J Proteome Res

January 2025

Discovery Research, AbbVie, Inc., 1 North Waukegan Rd., North Chicago, Illinois 60064, United States.

Affinity capture (AC) combined with mass spectrometry (MS)-based proteomics is highly utilized throughout the drug discovery pipeline to determine small-molecule target selectivity and engagement. However, the tedious sample preparation steps and time-consuming MS acquisition process have limited its use in a high-throughput format. Here, we report an automated workflow employing biotinylated probes and streptavidin magnetic beads for small-molecule target enrichment in the 96-well plate format, ending with direct sampling from EvoSep Solid Phase Extraction tips for liquid chromatography (LC)-tandem mass spectrometry (MS/MS) analysis.

View Article and Find Full Text PDF

Competitive fitness is a fundamental concept in evolutionary biology that captures the ability of organisms to survive, reproduce, and compete for resources in their environment. Competitive fitness is typically assessed in the lab by growing two or more competitors together and measuring the frequency of each at multiple time points. Traditional microbial competitive fitness assays are labor intensive and involve plating on solid medium and counting colonies.

View Article and Find Full Text PDF

We present a method for conjugating antigens to gold nanoparticles (GNPs) during their synthesis via gas plasma, eliminating the need for chemical linkers and significantly speeding up the process (taking only 15 min). This fast, linker-free method produces biocompatible and stable GNPs, with potential for immunotherapy applications, such as antigen and antibody conjugation and drug delivery. We demonstrate the conjugation of the antigen Nestin (NES), a tumor marker, to GNPs using two approaches.

View Article and Find Full Text PDF

In this study, we demonstrate MXene (TiCT)-based coin-cell asymmetric supercapacitor (coin-cell ASC) exhibiting high energy density and high power density along with good capacitance. We synthesized mesoporous carbon (MC) by annealing alginic acid at varying temperatures (900 °C, 1000 °C and 1100 °C). Among the prepared samples, MC-1000 exhibited a highly porous structure and a higher surface area.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!