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Extraction and Characterization of Inulin-Like Fructans from Hydroponically Grown Stevia rebaudiana Roots for Food Applications.
Plant Foods Hum Nutr
January 2025
Departamento de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Laboratório de Biotecnologia de Produtos Naturais e Sintéticos (LABIPROS), Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, Paraná, 87020-900, Brazil.
Stevia rebaudiana is a plant native to South America known for producing steviol glycosides and fructans used in low-calorie and functional foods. This study aimed to cultivate and isolate inulin from hydroponically grown S. rebaudiana roots.
View Article and Find Full Text PDFBeyond the baseline: Quantification of two Phosphatidylethanol (PEth) homologues in Whole Blood by LC-MS/MS and Retrospective Data Analysis from a National Reference Laboratory.
J Anal Toxicol
January 2025
ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, UT, USA.
Unlabelled: Background - Alcohol is the most abused substance in Western society, resulting in major economic losses and negative health consequences. Therefore, there is a need for a selective and robust detection method for alcohol consumption in various clinical and forensic settings. This study aimed to validate a mass spectrometry method for quantifying phosphatidylethanol (PEth) and perform retrospective data analysis from the patient population of a national reference laboratory.
View Article and Find Full Text PDFSalivary extracellular vesicles isolation methods impact the robustness of downstream biomarkers detection.
Sci Rep
December 2024
Sys2Diag, UMR9005 CNRS/ALCEN, Cap Gamma, Parc Euromédecine, 1682 Rue de la Valsière, CS 40182, 34184, Montpellier Cedex 4, France.
Extracellular vesicles (EVs), crucial mediators in cell-to-cell communication, are implicated in both homeostatic and pathological processes. Their detectability in easily accessible peripheral fluids like saliva positions them as promising candidates for non-invasive biomarker discovery. However, the lack of standardized methods for salivary EVs isolation greatly limits our ability to study them.
View Article and Find Full Text PDFHigh-Throughput Molecular Dynamics Study and Turbidity Analysis on Clustering and Agglomeration of Cellulose Nanocrystals in Suspensions.
Langmuir
January 2025
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, P. R. China.
The dispersion of cellulose nanocrystals (CNCs) in suspensions determines the quality of the CNC-reinforced composites. Before being mixed into the composite matrix, stable suspensions must maintain a well-dispersed state, requiring proper design strategies to prevent agglomeration and precipitation. Considering the volume fraction, aspect ratio, and zeta potential, this paper proposes a coarse-grained model to simulate CNC clustering and an experimental program to observe accelerated precipitation of CNCs.
View Article and Find Full Text PDFPlasma bile acid profile analysis by liquid chromatography-tandem mass spectrometry and its application in healthy subjects and IBD patients.
J Pharm Biomed Anal
December 2024
Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:
Bile acids (BAs), not only promote the absorption of fat-soluble nutrients and regulate the metabolism of multiple substances but also have a potential role as diagnostic and prognostic indicators in a variety of diseases such as cholestasis, hepatocellular carcinoma, and diabetes mellitus. Here, a rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of 50 BAs was developed and validated. Sample preparation included internal standard spiking, followed by protein precipitation, centrifugation, solvent evaporation, and reconstitution.
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