Bacillus anthracis is a spore-forming bacterium and the causative agent of the disease anthrax. The Oxford Protein Production Facility has been targeting proteins from B. anthracis in order to develop high-throughput technologies within the Structural Proteomics in Europe project. As part of this work, the structure of 5-formyltetrahydrofolate cyclo-ligase (BA4489) has been determined by X-ray crystallography to 1.6 A resolution. The structure, solved in complex with magnesium-ion-bound ADP and phosphate, gives a detailed picture of the proposed catalytic mechanism of the enzyme. Chemical differences from other cyclo-ligase structures close to the active site that could be exploited to design specific inhibitors are also highlighted.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330188 | PMC |
| http://dx.doi.org/10.1107/S1744309107007221 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reverse engineering of Onivyde® - Irinotecan liposome injection.
Int J Pharm
November 2024
Department of Pharmaceutical Sciences and the Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address:
Onivyde® is an intravenous irinotecan liposomal injection approved by the FDA for the treatment of gemcitabine-refractory metastatic adenocarcinoma of the pancreas in combination with fluorouracil and leucovorin. In the Onivyde® formulation, irinotecan is encapsulated in the inner compartment of the liposome using sucrose octasulfate as a trapping agent, and stabilized by a pegylated lipid membrane, resulting in prolonged circulation in the body. Due to its complex formulation design, there is limited information available regarding the critical quality attributes (CQAs) of Onivyde® and suitable methods for evaluating these attributes.
View Article and Find Full Text PDFPERIOPERATIVE FLOT CHEMOTHERAPY FOR GASTRIC CANCER: A RETROSPECTIVE SINGLE-CENTER COHORT TRIAL.
Georgian Med News
September 2024
Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.
Aim: to analyze the short- and long-term outcomes of surgical treatment of patients with gastric cancer who received perioperative chemotherapy (PCT) FLOT (fluorouracil, leucovorin, oxaliplatin and docetaxel).
Materials And Methods: A retrospective cohort study included 146 patients who received surgical treatment at the Faculty Surgery Clinic of Sechenov University for gastric cancer (GC) and/or EGJC Sievert Type II-III in the period from January 2018 to December 2022. The main group consisted of 28 patients who received PCT FLOT; there were 118 patients operated "up front" in control group.
Cell hiding in colorectal cancer: correlation with response to chemotherapy in vitro and in vivo.
Sci Rep
November 2024
Research Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod, Russia.
Article Synopsis
- Resistance to chemotherapy in colorectal cancer may be linked to cell-in-cell (CIC) structures, where one cell protects another from harmful treatments.
- Studies using advanced microscopy techniques showed that resistance to drugs like oxaliplatin and Irinotecan was associated with an increased formation of CIC structures in cancer cells.
- The findings suggest that CIC structures could serve as a potential marker for predicting treatment success in colorectal cancer, warranting further clinical research.
Impact of MTHFD2 Expression in Bladder/Breast Cancer and Screening of Its Potential Inhibitor.
ACS Omega
November 2024
Molecular Virology Laboratories, Department of Biosciences, COMSATS University Islamabad, Islamabad 45550, Pakistan.
Genes of folate-mediated 1 carbon metabolism are found to be highly upregulated in tumor cells and promote cancer cell proliferation. The current study aimed to determine the expression of the MTHFD2 gene in bladder and breast cancers. Furthermore, the determination of potential ligand-based inhibitors against MTHFD2 was performed in comparison with those of chemotherapeutic drugs and natural plant-based compounds.
View Article and Find Full Text PDFDendritic Molecular Baskets for Selective Binding of Toxic Methotrexate.
Angew Chem Int Ed Engl
November 2024
Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH-43210, USA.
We describe the preparation, assembly, recognition characteristics, and bioactivity of dendritic basket 6. This novel cavitand has a deep aromatic pocket with three (S)-glutamic acid dendrons at the rim to amplify water solubility and prevent self-association. H NMR spectroscopy, calorimetry (ITC), and mass spectrometry (ESI-MS) measurements validate the formation of an inclusion complex between 6 and anticancer drug methotrexate (MTX) in water (K=9.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!