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Scaffold-enabled high-resolution cryo-EM structure determination of RNA.
Nat Commun
January 2025
Department of Chemistry and Biochemistry, University of California, San Diego, CA, USA.
Cryo-EM structure determination of protein-free RNAs has remained difficult with most attempts yielding low to moderate resolution and lacking nucleotide-level detail. These difficulties are compounded for small RNAs as cryo-EM is inherently more difficult for lower molecular weight macromolecules. Here we present a strategy for fusing small RNAs to a group II intron that yields high resolution structures of the appended RNA.
View Article and Find Full Text PDFOptofluidic paper-based analytical device for discriminative detection of organic substances via digital color coding.
Microsyst Nanoeng
January 2025
Department of Chemical and Biomolecular Engineering, Chonnam National University, 50 Daehak-ro, Yeosu-si, Jeollanam-do, 59626, Republic of Korea.
Developing a portable yet affordable method for the discrimination of chemical substances with good sensitivity and selectivity is essential for on-site visual detection of unknown substances. Herein, we propose an optofluidic paper-based analytical device (PAD) that consists of a macromolecule-driven flow (MDF) gate and photonic crystal (PhC) coding units, enabling portable and scalable detection and discrimination of various organic chemical, mimicking the olfactory system. The MDF gate is designed for precise flow control of liquid analytes, which depends on intermolecular interactions between the polymer at the MDF gate and the liquid analytes.
View Article and Find Full Text PDFInsights into conformational ensembles of compositionally identical disordered peptidomimetics.
Polym Chem
August 2024
Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
While the conformational ensembles of disordered peptides and peptidomimetics are complex and challenging to characterize, they are a critical component in the paradigm connecting macromolecule sequence, structure, and function. In molecules that do not adopt a single predominant conformation, the conformational ensemble contains rich structural information that, if accessible, can provide a fundamental understanding related to desirable functions such as cell penetration of a therapeutic or the generation of tunable enzyme-mimetic architecture. To address the fundamental challenge of describing broad conformational ensembles, we developed a model system of peptidomimetics comprised of polar glycine and hydrophobic -butylglycine to characterize using a suite of analytical techniques.
View Article and Find Full Text PDFMPicker: visualizing and picking membrane proteins for cryo-electron tomography.
Nat Commun
January 2025
Key Laboratory for Protein Sciences of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing, China.
Advancements in cryo-electron tomography (cryoET) allow the structure of macromolecules to be determined in situ, which is crucial for studying membrane protein structures and their interactions in the cellular environment. However, membranes are often highly curved and have a strong contrast in cryoET tomograms, which masks the signals from membrane proteins. These factors pose difficulties in observing and revealing the structures of membrane proteins in situ.
View Article and Find Full Text PDFDrug in Drug: Quorum Sensing Inhibitor in Star-Shaped Antibacterial Polypeptides for Inhibiting and Eradicating Corneal Bacterial Biofilms.
ACS Nano
January 2025
Eye Center, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang Provincial Key Laboratory of Ophthalmology, Zhejiang Provincial Clinical Research Center for Eye Diseases, Zhejiang Provincial Engineering Institute on Eye Diseases, Hangzhou 310009, P. R. China.
Biofilm-related bacterial keratitis is a severe ocular infection that can result in drastic vision impairment and even blindness. However, the therapeutic efficiency of clinical antibiotic eyedrops is often compromised because the bacteria in the biofilms resist bactericide the community genetic regulation, namely, bacterial quorum sensing. Herein, quercetin (QCT)-loaded star-shaped antibacterial peptide polymer (SAPP), QCT@SAPP, is developed based on a "drug" in a "drug" strategy for inhibiting and eradicating biofilms on the cornea.
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