A fully automated procedure for detecting and centering protein crystals in the X-ray beam of a macromolecular crystallography beamline has been developed. A cryo-loop centering routine that analyzes video images with an edge detection algorithm is first used to determine the dimensions of the loop holding the sample; then low-dose X-rays are used to record diffraction images in a grid over the edge and face plane of the loop. A three-dimensional profile of the crystal based on the number of diffraction spots in each image is constructed. The derived center of mass is then used to align the crystal to the X-ray beam. Typical samples can be accurately aligned in approximately 2-3 min. Because the procedure is based on the number of ;good' spots as determined by the program Spotfinder, the best diffracting part of the crystal is aligned to the X-ray beam.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1107/S0909049507004803 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Laser surface nanoalloying of Fe, Si, and C on aluminum substrates with excellent optical and electronic properties.
Nanoscale Adv
January 2025
Energy Masteries Laboratory, Physics Department, School of Sciences and Engineering, The American University in Cairo New Cairo 11835 Egypt
Laser surface alloying of Fe, Si, and C on aluminium is demonstrated using a Q-switched Nd:YAG laser as the source of energy. The fundamental wavelength of the laser beam was 1064 nm with an output energy of 100 mJ and a pulse duration of 10 ns. The exposure was conducted in repetitive mode with a frequency rate of 1 Hz.
View Article and Find Full Text PDFEffects of X-ray and electron beam irradiation on wine quality: Emphasizing phenolic compounds and aroma profiles.
Food Chem X
January 2025
College of Enology, Northwest A&F University, Shaanxi 712100, China.
The content of flavor compounds in wine is limited by factors such as climate warming and the resistance of cell walls to maceration. This study used X-rays (ionizing radiation) and electron beams (particle radiation) at 0.5, 2, and 7 kGy for grape pre-treatment before winemaking.
View Article and Find Full Text PDFReduction of Streak Artifacts in the Superior Vena Cava for Better Visualization of Mediastinal Structures Through Virtual Monoenergetic Reconstructions Using a Photon-counting Detector Computed Tomography.
J Thorac Imaging
January 2025
Department of Radiology and Nuclear Medicine, University Medical Centre Mannheim.
Purpose: Computed tomography (CT) is crucial in oncologic imaging for precise diagnosis and staging. Beam-hardening artifacts from contrast media in the superior vena cava can degrade image quality and obscure adjacent structures, complicating lymph node assessment. This study examines the use of virtual monoenergetic reconstruction with photon-counting detector CT (photon-counting CT) to mitigate these artifacts.
View Article and Find Full Text PDF[Surgical Management of Metastatic Spinal Tumors].
No Shinkei Geka
January 2025
Department for Neurosurgery, Aichi Cancer Center.
Historically, metastatic spinal tumors have been treated using open spinal fixation, invasive decompressive techniques, and low-dose palliative conventional external beam radiotherapy. As patients with metastatic disease are now living longer, the need for long-term local tumor control is becoming important. Spine stereotactic body radiotherapy has emerged as a valuable alternative option to achieve long-term local tumor control by delivering high doses of radiation to tumors and sparing the spinal cord.
View Article and Find Full Text PDFThe diagnostic performance and optimal strategy of cone beam CT-assisted bronchoscopy for peripheral pulmonary lesions: A systematic review and meta-analysis.
Pulmonology
December 2025
Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
Cone-beam computed tomography (CBCT) assisted bronchoscopy shows prospective advantages in diagnosing peripheral pulmonary lesions (PPLs), but its diagnostic value and potential influencing factors remain unclear. What is the clinical value and optimal strategy of CBCT-assisted bronchoscopy in diagnosing PPLs? The references were searched from PubMed, EmBase, and Web of Science. Studies reporting diagnostic yield and potential influencing factors of CBCT-assisted bronchoscopy were included.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!