AI Article Synopsis
- Baculoviruses are effective in controlling insect populations, typically through ingestion, but spiracular infection methods were evaluated for virus production using different forms of Helicoverpa armigera nucleopolyhedrovirus.
- Spiracular treatments led to larval deaths, but the diet surface treatment method resulted in higher mortality and better virus yield, especially with intact polyhedral inclusion bodies (PIB).
- Despite comparable PIB yield per larva in spiracle methods, the overall low mortality and higher labor needed make it less practical than the standard diet treatment for large-scale virus production.
Article Abstract
Baculoviruses are important microbial control agents of insects, with per os mode of infectivity. However, recently the spiracular infection of this virus group was suggested as an optimum method for virus production in grown up larvae. In this regard, a detailed evaluation of the spiracular infection with intact polyhedral inclusion bodies (PIB), alkali liberated virions and alkali liberated filtered virions of Helicoverpa armigera (Hubner) nucleopolyhedrovirus at 1 x 10(8), 1 x 10(7) and 2 x 10(6)PIB/ml concentrations was undertaken and compared with the standard diet surface treatment method. All the spiracle treatments resulted in larval death due to virus infection with alkali liberated virions causing higher mortality of larvae than alkali liberated filtered virions and intact PIB. Diet surface treatment method resulted in very high mortality as compared to spiracle treatment and among the different inoculum tested the intact PIB resulted in higher larval mortality. The PIB yield/larva in spiracle treatment was comparable with the diet surface treatment method, but due to very low larval mortality it resulted in low virus yield/100 inoculated larvae. Diet surface treatment with 5 x 10(7)PIB/ml concentration of virus resulted in the maximum yield of PIB/100 inoculated larvae. Low mortality, higher labour requirement and low amenability for mechanization for spiracle treatment method make it unviable for mass production of the virus in large scale compared to the standard diet surface treatment method.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jviromet.2007.01.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of Probiotics and Diet Management in Patients With Irritable Bowel Syndrome: A Systematic Review and Network Meta-analysis.
Nutr Rev
January 2025
Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu, Sichuan 610041, China.
Context: The efficacy of probiotics and diet management in irritable bowel syndrome (IBS) is controversial, and their relative effectiveness remains unclear.
Objective: This study aimed to evaluate the effects of probiotics, diet management, and their combination on IBS.
Data Sources: PubMed, Embase, Cochrane, and Web of Science were searched from inception to July 10, 2023, for relevant studies, including symptom relief, IBS-symptom severity score (-SSS), and IBS-quality of life measure (-QOL).
Molecular Mechanisms of Dietary Compounds in Cancer Stem Cells from Solid Tumors: Insights into Colorectal, Breast, and Prostate Cancer.
Int J Mol Sci
January 2025
Department of Biochemistry and Biophysics, "Carol Davila" University of Medicine and Pharmacy of Bucharest, 050474 Bucharest, Romania.
Cancer stem cells (CSC) are known to be the main source of tumor relapse, metastasis, or multidrug resistance and the mechanisms to counteract or eradicate them and their activity remain elusive. There are different hypotheses that claim that the origin of CSC might be in regular stem cells (SC) and, due to accumulation of mutations, these normal cells become malignant, or the source of CSC might be in any malignant cell that, under certain environmental circumstances, acquires all the qualities to become CSC. Multiple studies indicate that lifestyle and diet might represent a source of wellbeing that can prevent and ameliorate the malignant phenotype of CSC.
View Article and Find Full Text PDFMicroencapsulation of Deer Oil in Soy Protein Isolate-Chitosan Complex Coacervate-Preparation, Characterization, and Simulated Digestion.
Foods
January 2025
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Deer oil (DO) is a potentially beneficial functional oil; however, its sensitivity to environmental factors (e.g., oxygen and heat), difficulty in transport, and unfavorable taste hinder practical use.
View Article and Find Full Text PDFHepatocellular CMPK2 promotes the development of metabolic dysfunction-associated steatohepatitis.
J Hepatol
January 2025
State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China; Department of Pharmacy, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Center for Innovative Traditional Chinese Medicine Target and New Drug Research, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, China. Electronic address:
Background & Aims: Metabolic dysfunction-associated steatohepatitis (MASH), a progressive subtype of metabolic dysfunction-associated steatotic liver disease (MASLD), has limited pharmacological treatment options. Therefore, we aimed to identify novel therapeutic targets.
Methods: The Gene Expression Omnibus (GEO) database and human liver tissues obtained from patients with MASH were used to identify differentially expressed genes in MASH.
Optimizing TMS dosimetry: evaluating the effective electric field as a novel metric.
Phys Med Biol
January 2025
Department of Information Engineering, Electronics and Telecommunications (DIET) , University of Rome La Sapienza, Via Eudossiana 18, Rome, 00184, ITALY.
Objective: This study introduces the effective electric field (Eeff) as a novel observable for transcranial magnetic stimulation (TMS) numerical dosimetry. Eeff represents the electric field component aligned with the local orientation of cortical and white matter neuronal elements. To assess the utility of Eeff as a predictive measure for TMS outcomes, we evaluated its correlation with TMS induced muscle responses and compared it against conventional observables, including the electric (E-)field magnitude, and its components normal and tangential to the cortical surface.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!