An amphiphilic homopolymer scaffold has been used to bind to the protein, cytochrome c. This interaction is analyzed using cyclic voltammetry, native gel electrophoresis, UV-visible absorption, and circular dichroism spectroscopy. The polymer binds to cytochrome c with micromolar affinity and the association of polymer with cytochrome c leads to a structural change of the protein. This conformational change exposes the heme unit of the protein, which affords an opportunity to reversibly modulate its electron-transfer properties. We have also shown that the electrostatic binding of polymer to cytochrome c can be used to disrupt its interaction with its natural partner, cytochrome c peroxidase.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/la063063p | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nonionic Amphiphilic Copolymers of Poly(poly(ethylene Glycol) Methacrylate) Brushes with Methyl Methacrylate Prepared by Atom Transfer Radical Polymerization as Dry Solid Polymer Electrolytes for Next Generation Li-ion Battery Applications.
ACS Appl Energy Mater
December 2024
Department of Chemical and Environmental Process Engineering, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest H-1111, Hungary.
Amphiphilic copolymers of comb-like poly(poly(ethylene glycol) methacrylate) (PPEGMA) with methyl methacrylate (MMA) synthesized by one-pot atom transfer radical polymerization were mixed with lithium bis (trifluoromethanesulfonyl) imide salt to formulate dry solid polymer electrolytes (DSPE) for semisolid-state Li-ion battery applications. The PEO-type side chain length (EO monomer's number) in the PEGMA macromonomer units was varied, and its influence on the mechanical and electrochemical characteristics was investigated. It was found that the copolymers, due to the presence of PMMA segments, possess viscoelastic behavior and less change in mechanical properties than a PEO homopolymer with 100 kDa molecular weight in the investigated temperature range.
View Article and Find Full Text PDFUnusual Endotaxy Growth of Hexagonal Nanosheets by the Self-Assembly of a Homopolymer.
Angew Chem Int Ed Engl
December 2024
Department of Gynaecology and Obstetrics, Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Clinical Research Center for Anesthesiology and Perioperative Medicine, Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China.
A classical crystallization usually grows epitaxially from a crystal nucleus. Presented in this study is an unusual endotaxy growth manner of a crystalline homopolymer to form hexagonal nanosheets. The amphiphilic homopolymer, poly(3-(4-(phenyldiazenyl)phenoxy)propyl methacrylate) (PAzoPMA), is first annealed in isopropanol to afford a hexagonal nut-like structure.
View Article and Find Full Text PDFCholic-Acid Derived, Guanidine-Functionalized Polymers as Broad-Spectrum Antimicrobial Agents.
ACS Omega
December 2024
School of Chemistry, The University of Melbourne, Parkville, Victoria 3010, Australia.
In this study, we report the design of a new guanylated, cholic-acid-based monomer (GM) to combat antimicrobial resistance. The microbial activity stems from the interfacial amphiphilicity of cholic acid, while guanidine shows a strong association with phosphate, which promotes binding to membrane phospholipids. The monomer showed strong antimicrobial activity; however, surprisingly, homopolymers synthesized by photoiniferter reversible addition-fragmentation chain-transfer (RAFT) polymerization of GM completely lost their activity likely due to the conformation of the polymer.
View Article and Find Full Text PDFSynthesis of Amphiphilic Cationic Poly(β-amino acid) Derivatives and Their PEG Length Optimization for mRNA Transfection.
Biomacromolecules
November 2024
Department of Biological Sciences and Bioengineering, Inha University, 100 Inha-ro, Michuhol-gu, Incheon 22212, Republic of Korea.
We synthesized a series of amphiphilic cationic poly(ethylene glycol)--poly(β-amino acid) derivatives with various lengths of PEG to investigate the effects of PEG lengths on mRNA transfection. The surface charges of the polyplexes with a DP ≥ 4 gradually decreased as DP increased. This indicated that hydrophilic PEG with a DP ≥ 4 was exposed on the surface of the polyplexes, which was further confirmed using H NMR.
View Article and Find Full Text PDFHydrophobic fouling-resistant electrospun nanofiber membranes from poly(vinylidene fluoride)/polyampholyte blends.
Soft Matter
November 2024
Department of Physics & Astronomy, Tufts University, Medford, MA 02155, USA.
This study reports the fabrication of non-woven fibrous membranes from electrospinning blended solutions of PVDF with polyampholytes in -dimethylformamide and methanol. Polyampholytes are macromolecules that have both positive and negative charged units in different side groups attached to the backbone. In this study, we used a random polyampholyte amphiphilic copolymer (r-PAC) synthesized by co-polymerizing a hydrophobic monomer in addition to the positive and negative charged monomer units, to reduce the fouling propensity of PVDF electrospun membranes while preserving its inherent hydrophobicity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!