AI Article Synopsis
- Achieved complete regioselective separation of five pairs of isomeric dipalmitoyl polyalkenoyl glycerols using RP-HPLC on a single ODS column.
- Testing involved four ODS columns with varying percentages of free silanol groups, adapting binary mobile phases of different solvents based on the stationary phase's properties.
- The study found that polar solvents worked better with stationary phases high in free silanol groups, and isomer retention was influenced by chain length, double bond count, and acyl chain position in the glycerol molecule.
Article Abstract
Complete regioselective separation of five pairs of isomeric dipalmitoyl polyalkenoyl glycerols with two to six double bonds in the unsaturated acyl residues has been achieved by RP-HPLC on a single ODS column. Four ODS columns with stationary phases containing different percentages of free silanol groups have been tested. Binary mobile phases of ACN admixed with dichloromethane, tetrahydrofuran, 1-propanol, 2-propanol, ethanol, or acetone have been examined. The choice of modifier depended on the nature of the stationary phase. The more polar solvents were better suited for stationary phases with higher percentage of free silanol groups. Isomeric species were eluted according to chain length, number of double bonds, and the position of the unsaturated acyl chain in the glycerol molecule. Retention increases in the order 20:5 < 22:6 < 18:3 < 20:4 << 18:2. Within each isomeric pair, the species with unsaturated acyl chain occupying either the sn-1- or the 3-position were retained preferentially. Complete simultaneous regioselective separation of 10 isomeric triacylglycerols in a single isocratic run on a single ODS column was demonstrated.
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| http://dx.doi.org/10.1002/jssc.200500504 | DOI Listing |
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