Introduction: Granulosa cell tumour is uncommon malignant tumor which belongs to the sexual cord-stromal tumors of the ovary. It arises from the specialized stromal cells of the ovary. These tumors are unilateral in 95%. Over 90% of patients survive more than 10 years after surgery, but the recurrence may appear even 25 years after surgery.
Case Report: The authors present a 54-year-old woman who was admitted for abdominal pain and ultrasonography revealed tumor in the abdomen. During the surgery, a spheric, well circumscribed tumor of the mesentery, close to the forth portion of duodenum, was excised. Histological and immunohistochemical examinations verified that it was the metastasis of the granulosa cell tumor of the ovary that had been removed 19 years ago when salpingo-oophorectomy had been performed. Postoperative recovery was uneventful. Twenty six months later, the tumor 4 cm in diameter recurred, and was localized to the left of duodenum, close to the inferior mesenteric artery, which was perfectly ideally, together with fat tissue and surrounding lymph nodes. She has remained symptom free so far.
Conclusion: To the best of our knowledge, this is the first case of the isolated metastasis of the granulosa cell tumor in the mesentery, that appeared 19 years after original surgery.
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http://dx.doi.org/10.2298/sarh0612546c | DOI Listing |
Proc Natl Acad Sci U S A
February 2025
Pediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, MA 02114.
Anti-Müllerian hormone (AMH) protects the ovarian reserve from chemotherapy, and this effect is most pronounced with Doxorubicin (DOX). However, DOX toxicity and AMH rescue mechanisms in the ovary have remained unclear. Herein, we characterize the consequences of these treatments in ovarian cell types using scRNAseq.
View Article and Find Full Text PDFJ Assist Reprod Genet
January 2025
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, Sassari, Italy.
Purpose: This study aimed to evaluate the effectiveness of single versus group culture strategies for cumulus-oocyte complexes (COCs) derived from early antral follicles (EAFs), with the goal of optimizing culture conditions to increase oocyte availability for assisted reproductive technologies.
Methods: COCs isolated from EAFs (350-450 µm) from sheep ovaries were cultured in TCM199 medium supplemented with 0.15 µg/mL Zn as zinc sulfate, 10 IU/mL FSH, 10 ng/mL estradiol, 50 ng/mL testosterone, 50 ng/mL progesterone, and 5 µM Cilostamide.
Life Med
February 2024
Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Ovarian aging is mainly characterized by a progressive decline in oocyte quantity and quality, which ultimately leads to female infertility. Various therapies have been established to cope with ovarian aging, among which exosome-based therapy is considered a promising strategy that can benefit ovarian functions via multiple pathways. Here, we isolated and characterized exosomes derived from ovarian follicular fluid and profiled the differential expression patterns of noncoding exosomal RNAs in young and aged women.
View Article and Find Full Text PDFJ Assist Reprod Genet
January 2025
Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Clinical Sciences, Research Group Genetics, Reproduction and Development, Centre for Medical Genetics, Laarbeeklaan 101, 1090, Brussels, Belgium.
Purpose: Primary ovarian insufficiency (POI) is an important cause of female infertility, stemming from follicle dysfunction or premature oocyte depletion. Pathogenic variants in genes such as NOBOX, GDF9, BMP15, and FSHR have been linked to POI. NOBOX, a transcription factor expressed in oocytes and granulosa cells, plays a pivotal role in folliculogenesis.
View Article and Find Full Text PDFMol Cell Endocrinol
January 2025
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA; Reproductive Medicine Associates of New York, Department of Obstetrics, Gynecology and Reproductive Science, Division of Reproductive Endocrinology and Infertility, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
The purpose of this study was to examine the deposition of advanced glycation end products (AGEs) and their receptors, RAGE, in ovarian follicles during folliculogenesis in mice fed high (H-AGE) or low (L-AGE) AGE diets and following superovulation with gonadotropins. We hypothesize that H-AGE diet is associated with increased AGE deposition and RAGE expression in various stages of ovarian follicular development, and superovulation with gonadotropins may alter these changes. C57BL/6J mice were fed low L-AGE (n=10) or H-AGE (n=10) diet for 12 weeks.
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