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K. marxianus was cultivated under conditions of PEF (pulse electric field) action and a selenium source. The culture duration after which cells were treated with PEF, the field exposure time and the selenium concentration in the medium were all optimized. Optimization of culture duration caused a 33% increase in selenium accumulation in cells as compared to the control with no PEF treatment. The highest selenium accumulation--about 167 microg/g dry mass (DM)--was recorded after 3-minute PEF treatment of 16-hour culture. A roughly two-fold increase in selenium content was achieved after optimization of culture duration and PEF treatment time. Finding the optimum selenium concentration in the medium brought about a 13-fold increase of selenium accumulation in the cells of K. marxianus.
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