[Inhibiting E7 oncogene in CaSki cells of cervical cancer by vector-based RNA interference technique].

Objective: To test of the effect of vector-based RNA interference (RNAi) technique on inhibiting HPV16E7 gene in CaSki cells of cervical cancer.

Methods: The HPV16E7-specific siRNA expression vectors P1, P2 and P3 were constructed and transfected into CaSki cells by liposome. The expression of HPV16E7 mRNA and protein were detected by real-time RT-PCR and Western blot.

Results: The expression of HPV16E7 mRNA and protein decreased with the transfection of P1, P2 and P3. Vector P1 had the strongest inhibition effect, with an inhibition rates of 92.86% and 81.0% for the expression of HPV16E7 mRNA and protein respectively three weeks after transfection.

Conclusion: The expression of E7 gene in CaSki cells can be inhibited by HPV16E7-specific siRNA expression vector.