[Vitro culture of blastocystis hominis in medium DMEM].

Objective: To establish the vitro culture of Blastocystis hominis (B. h) in medium DMEM for the further research on diagnosis, life cycle and pathogenicity of this intestinal protoza.

Methods: The growth, reproduction and relevant factors of B . h under different culture conditions including sorts and concentrations of serum, pHs and number of inoculation were compared.

Results: Conditions for the continuously anaerobic culture of B. h in medium DMEM were as follows: the number of inoculation were no less than 10(5) cells per tube, pHs ranged 7.0 - 8.0, concentrations of calf serum (or human serum and horse serum) ranged 10% - 30% , antibiotics and Amphotericin B should be added, subculturing could be choose at the each peaking-day 3,6 or 5 at 37 degrees C.

Conclusion: The medium DMEM could be used in diagnosis and continuously vitro culture for B. h.