Objective: To clone novel gene fragments differentially expressed from diabetic rats supplemented with zinc and to detect their expression distribution in various tissues.
Methods: cDNA fragments from former research project were cloned, sequenced and BLASTn analysed. The RT-PCR of the two novel genes were made using the primers designed according to the sequence of cDNA to observe the expression changes in liver of various groups and their expression distributions in various tissues.
Results: 2 # cDNA and 6 # cDNA were shown to be the novel gene fragments for no matched gene with them in GenBank. The expression level of DM group and DM + Zn group was obviously lower than those of NC group. The expression level of DM + Zn group was higher than those of DM group ( P < 0.05) . The expression level of 2 # cDNA was higher in brain and pancreas, lower in kidney, and none in heart, muscle and thymus. The expression of 6 # cDNA can be found in heart, muscle, brain, kidney and pancreas, but thymus. 2 # cDNA and 6 # cDNA were registered to GenBank with the numbers of AY952968 and AY952970 respectively.
Conclusion: Two novel genes differentially expressed in diabetic rats supplemented with zinc were cloned and expressed in various tissues, and were successfully submitted to GenBank.
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