AI Article Synopsis
- The study aimed to find genes linked to radioresistance in oral squamous cell carcinoma (OSCC) by comparing gene expression in radioresistant and radiosensitive cell lines after X-ray exposure.
- A microarray analysis found 167 genes overexpressed in radioresistant cells, with 40 genes fitting into significant genetic networks related to cancer functions, including growth and adhesion.
- Real-time PCR confirmed that these 25 cancer-related genes were consistently higher in radioresistant cells, suggesting they could serve as markers for tailoring radiotherapy in OSCC.
Article Abstract
To identify genes associated with radioresistant oral squamous cell carcinoma (OSCC), we compared gene expression signatures between OSCC cell lines exhibiting radioresistance and cells with radiosensitivity after X-ray irradiation in a dose-dependent manner using Affymetrix GeneChip analysis with Human Genome-U133 plus 2.0 GeneChip. The microarray data identified 167 genes that were significantly overexpressed in radioresistant cells after X-ray irradiation. Among the genes identified, 40 were mapped to 3 highly significant genetic networks identified by the Ingenuity Pathway Analysis tool. Gene ontology analysis showed that cancer-related function had the highest significance. The 40 genes included 25 cancer-related genes that formed 1 network and were categorized by function into growth and proliferation, apoptosis, and adhesion. Furthermore, real-time quantitative reverse transcriptase-polymerase chain reaction showed that the mRNA expression levels of the 25 genes were higher in radioresistant cells than in radiosensitive cells in a dose-dependent manner and in a time-dependent manner. Our results suggest that the identified genes help to elucidate the molecular mechanisms of the radioresistance of OSCC and could be radiotherapeutic molecular markers for choosing the appropriate radiotherapy for this disease.
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| http://dx.doi.org/10.1002/ijc.22561 | DOI Listing |
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