Understanding the local CNS immune response to neoplasms is essential in the development of immune-based treatments for malignant brain tumors. Using rodent glioma models, we have recently found tumor-associated microglia/macrophages (MG/MP) to be less responsive to known MG/MP activators such as CpG, LPS and IFN-gamma. To understand the mechanism of MG/MP suppression, nuclear extracts from rodent intracranial C6 gliomas, C6 glioma-associated MG/MP, normal brain, and normal MG/MP were obtained and studied using Electrophoretic Mobility Shift Assay (EMSA). Among the nuclear factors studied (AP-1, IRF, USF-1 and Stat-1) only USF-1, which is constitutively expressed in most cells, was down-regulated in tumor-associated MG/MP, but not normal MG/MP. Because tumor-associated MG/MP had higher expression of IL-10 (but not TNF-alpha or TGF-beta), we evaluated the role of USF-1 on IL-10 expression. siRNA mediated inhibition of USF-1 expression in primary MG/MP cultures resulted in up-regulation of IL-10 mRNA but not TNF-alpha or TGF-beta. These findings suggest that USF-1 may play a role in IL-10 regulation in MG/MP in brain tumors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jneuroim.2006.12.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dose effect of corticosteroids on peripheral lymphocyte profiles in patients with systemic lupus erythematosus.
Clin Rheumatol
January 2025
Department of Rheumatology and Immunology, Peking University People's Hospital, 11 Xizhimen South Street, Beijing, 100044, China.
Objective: To investigate the dose effect of methylprednisolone (MP) on peripheral lymphocyte profiles in patients with systemic lupus erythematosus (SLE). This study investigated the impact of varied MP doses on peripheral lymphocyte subtypes in SLE patients.
Methods: We conducted a prospective study involving 51 SLE patients, categorized into four groups (40 mg/day, 80 mg/day, 500 mg/day, and 1000 mg/day) based on the administered MP dosage during hospitalization.
First-in-human phase 1 dose-escalation results with livmoniplimab, an antibody targeting the GARP:TGF-ß1 complex, as monotherapy and in combination with the anti-PD-1 antibody budigalimab in patients with advanced solid tumors.
Front Oncol
October 2024
New Experimental Therapeutics (NEXT) Oncology, San Antonio, TX, United States.
Article Synopsis
- Transforming growth factor (TGF)-ß1 is known to encourage tumor growth while also suppressing the immune system's ability to fight tumors; its latent form is associated with glycoprotein-A repetition predominant (GARP) on regulatory T cells.
- Livmoniplimab is a monoclonal antibody that targets the GARP:TGF-ß1 complex to impede the activation and release of TGF-ß1, and it's being studied in combination with another antibody, budigalimab, in a clinical trial for patients with advanced solid tumors.
- In the trial, 57 patients were treated with dosages ranging up to 1500mg, with manageable side effects like fatigue and nausea reported; while no
Article Synopsis
- - The Progesterone Breast Endometrial Safety Study aims to compare the safety of micronised progesterone (mP) to synthetic progestins (norethisterone acetate) in hormone therapy for menopausal women, focusing on breast and endometrial health.
- - Conducted at three university hospitals in Sweden, the study consists of two parts: the first assesses breast safety in a double-blind trial with 260 women, while the second evaluates endometrial safety with an additional 260 women using mP.
- - The research has ethical approval and results will be shared in scientific publications and meetings, with a total of 520 participants in both phases combined.
The particle effect: comparative toxicity of chlorpyrifos in combination with microplastics and phytoplankton particles in mussel.
Aquat Toxicol
October 2024
Centro Oceanográfico de Murcia, Instituto Español de Oceanografía (COMU-IEO), CSIC, Calle Varadero 1., San pedro del Pinatar, Murcia 30740, Spain. Electronic address:
Lately, the role of microplastics (MP) as vectors for dissolved contaminants and as vehicle for their transfer to aquatic organisms has received attention. Similarly to MP, other inorganic and organic particles may act as passive samplers. However, limited comparative knowledge exists at this respect.
View Article and Find Full Text PDFMicroplastics from face mask impairs sperm motility.
Mar Pollut Bull
June 2024
The Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China; University of Chinese Academy of Sciences, Beijing, China; Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Key Laboratory of Brain Connectome and Manipulation, Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, China. Electronic address:
Article Synopsis
- The COVID-19 pandemic has led to increased plastic pollution, particularly from single-use face masks, impacting coastal and marine environments.
- The study revealed that while feeding mice microplastics from masks at different doses for 21 days didn't affect overall health, it did harm sperm motility, especially at higher doses.
- The research indicates that these microplastics disrupt critical biological pathways related to reproduction, emphasizing the urgent need for better plastic waste management and eco-friendly alternatives.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!